Effect of Magnetic Field Exposure on Anchorage-Independent Growth of a Promoter-Sensitive Mous Epidermal Cell Line (JB6)

Details

• Journal Article:
  Environmental Health Perspectives
• Personal Author:
  Snawder, John E. ; Edwards, Richard M. ; Conover, David L. ; Lotz, W. Gregory
• Description:
  The anchorage-independent growth of mouse epidermal cells (JB6) exposed to 60-Hz magnetic fields (MF) was investigated. Promotion-responsive JB6 cells were suspended in agar (104 cells/plate) and exposed continuously to 0.10 or 0.96 mT, 60-Hz magnetic fields for 10-14 days, with or without concurrent treatment with the tumor promoter tetradecanoylphorbol acetate (TPA). Exposures to MF were conducted in a manner such that the experimenter was blind to the treatment group of the cells. At the end of the exposure period, the anchorage-independent growth of JB6 cells on soft agar was examined by counting the number of colonies larger than 60 µm (minimum of 60 cells). The use of a combined treatment of the cells with both MF and TPA was to provide an internal positive control to estimate the success of the assay and to allow evaluation of co-promotion. Statistical analysis was performed by a randomized block design analysis of variance to examine both the effect of TPA treatment (alone and in combination with MF exposure) and the effect of intra-assay variability. Transformation frequency of JB6 cells displayed a dose-dependent response to increasing concentrations of TPA. Coexposure of cells to both TPA and 0.10 or 0.96 mT, 60-Hz MF did not result in any differences in transformation frequency for any TPA concentrations tested (0-1 ng/ml). These data indicate that exposure to a 0.10 or 0.96 mT, 60-Hz MF does not act as a promoter or co-promoter in promotion-sensitive JB6 cell anchorage-independent growth. [Description provided by NIOSH]
• Subjects:
  Carcinogens Cell Division Cell Line, Transformed Cells, Cultured Cell Transformation, Neoplastic Clone Cells Cocarcinogenesis Dose-Response Relationship, Drug Electromagnetic Fields Epidemiology Epidermis Epithelial Cells Laboratories Magnetic Fields Mice Neoplasms Neoplasms, Radiation-Induced Occupational Health Safety Tetradecanoylphorbol Acetate

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• Keywords:
  Magnetic-fields; Magnetic-properties; Statistical-analysis; Cancer; Epidemiology; Animal-studies; Animals; Laboratory-animals; Cell-cultures; Cell-damage; Cell-function
• Source:
  Environ Health Perspect 1999 Mar; 107(3):195-198
• ISSN:
  0091-6765
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  4 pdf pages
• Volume:
  107
• Issue:
  3
• NIOSHTIC Number:
  nn:20027720
• Contact Point Address:
  J.E. Snawder, Taft Laboratories, MS-C23, National Institute for Occupational Safety and Health, 4676 Columbia Parkway, Cincinnati, OH 45226 USA
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Collection(s):
  National Institute for Occupational Safety and Health CDC Public Access
• Main Document Checksum:
  urn:sha-512:43c9505de24501759d7328183cadfbf68dcee129a4812fe8c1f4f5d43fd03a2c01796e78d0d64afd60859985d9acde9cb9add37ab2bf2b15aecf90eb2946cd97
• Download URL:
  https://stacks.cdc.gov/view/cdc/3968/cdc_3968_DS1.pdf
• File Type:
  [PDF - 1.42 MB ]