Storage Effects on Sample Integrity of Environmental Surface Sampling Specimens with Bacillus anthracis Spores

Details

• Alternative Title:
  Biosafety (Los Angel)
• Personal Author:
  Perry, K. Allison ; O’Connell, Heather A. ; Rose, Laura J. ; Noble-Wang, Judith A. ; Arduino, Matthew J.
• Description:
  The effect of packaging, shipping temperatures and storage times on recovery of Bacillus anthracis. Sterne spores from swabs was investigated. Macrofoam swabs were pre-moistened, inoculated with Bacillus anthracis spores, and packaged in primary containment or secondary containment before storage at -15°C, 5°C, 21°C, or 35°C for 0-7 days. Swabs were processed according to validated Centers for Disease Control/Laboratory Response Network culture protocols, and the percent recovery relative to a reference sample (T0) was determined for each variable. No differences were observed in recovery between swabs held at -15° and 5°C, (p ≥ 0.23). These two temperatures provided significantly better recovery than swabs held at 21°C or 35°C (all 7 days pooled, p ≤ 0.04). The percent recovery at 5°C was not significantly different if processed on days 1, 2 or 4, but was significantly lower on day 7 (day 2 vs. 7, 5°C, 10(2), p=0.03). Secondary containment provided significantly better percent recovery than primary containment, regardless of storage time (5°C data, p ≤ 0.008). The integrity of environmental swab samples containing Bacillus anthracis spores shipped in secondary containment was maintained when stored at -15°C or 5°C and processed within 4 days to yield the optimum percent recovery of spores.
• Subjects:
  Article Bacillus Anthracis Environmental Sampling QPCR Shipping Integrity Swab
• Source:
  Biosafety (Los Angel). 2013(Suppl 1).
• Pubmed ID:
  27213119
• Pubmed Central ID:
  PMC4870600
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  2013
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:2c788b6bd9d20216869066380c5cac68e2f1fb77d6e3093dd1c72d28bafe1502
• Download URL:
  https://stacks.cdc.gov/view/cdc/39638/cdc_39638_DS1.pdf
• File Type:
  [PDF - 218.95 KB ]