Details:

Alternative Title:
Am J Clin Nutr
Publisher's site:
http://dx.doi.org/10.3945/ajcn.114.091785
Personal Author:
Lakoff, Alanna ; Fazili, Zia ; Aufreiter, Susanne ; Pfeiffer, Christine M ; Connolly, Bairbie ; ... More +
Lakoff, Alanna ; Fazili, Zia ; Aufreiter, Susanne ; Pfeiffer, Christine M ; Connolly, Bairbie ; Gregory, Jesse F ; Pencharz, Paul B ; O’Connor, Deborah L ; Less -
Description:
Background Folate intakes that do not meet or greatly exceed requirements may be associated with negative health outcomes. A better understanding of contributors that influence the input side will help establish dietary guidance that ensures health benefits without associated risks. Colonic microbiota produce large quantities of folate, and [13C5]5-formyltetrahydrofolate infused during colonoscopy is absorbed. However, it is unclear if significant quantities of folate are absorbed in an intact microbiome. Objective We determined whether and how much of a physiologic dose of [13C5]5-formyltetrahydrofolate delivered in a pH-sensitive enteric caplet to an intact colonic microbiome is absorbed. Design Healthy adults ingested a specially designed pH-sensitive acrylic copolymer–coated barium sulfate caplet that contained 855 nmol (400 μg) [13C5]5-formyltetrahydrofolate. After a washout period ≥4 wk, subjects received an intravenous injection of the same compound (214 nmol). Serially collected blood samples before and after each test dose were analyzed by using a microbiological assay and liquid chromatography–tandem mass spectrometry. Results Caplet disintegration in the colon was observed by fluoroscopic imaging for 6 subjects with a mean (±SD) complete disintegration time of 284 ± 155 min. The mean (±SEM) rate of appearance of [13C5]5-methyltetrahydrofolate in plasma was 0.33 ± 0.09 (caplet) and 5.8 ± 1.2 (intravenous) nmol/h. Likely because of the significant time in the colon, the mean apparent absorption across the colon was 46%. Conclusions Folate is absorbed across the colon in humans with an undisturbed microbiome. This finding and previous observations of the size of the colonic depot of folate and its potential for manipulation by diet (eg, dietary fiber, oligosaccharides, and probiotics) suggest that an individual’s dietary folate requirement may differ depending on the consumption of dietary constituents that affect the size and composition of their gastrointestinal microbiota. In addition, a systematic investigation of the role of colonic folate on gastrointestinal development and the prevention of colorectal cancer is warranted. This trial was registered at clinicaltrials.gov as NCT00941174.
Subject:
[+]
Adolescent
Adult
Aged
Article
Carbon Isotopes
Chromatography, Liquid
Colon
Diet
Dose-Response Relationship, Drug
Drug Compounding
Female
Healthy Volunteers
Humans
Hydrogen-Ion Concentration
Leucovorin
Male
Middle Aged
Tandem Mass Spectrometry
Young Adult

Source:
Am J Clin Nutr. 100(5):1278-1286 ;
Pubmed ID:
25332326
Pubmed Central ID:
PMC4823011
Document Type:
Journal Article ;
Funding:
CC999999/Intramural CDC HHS/United States ; R01 DK072398/DK/NIDDK NIH HHS/United States ;
CC999999/Intramural CDC HHS/United States ; R01 DK072398/DK/NIDDK NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:9f8cfe4570e52b3366b6d5ccf0ca0b0bc556611d04c7e3fe67dc6d87d1b6b1db
File Type:

Supporting Files

• 	NIHMS768673-supplement-Supplemental_table_1.pdf	pdf
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