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Laboratory diagnosis of amebiasis : Entamoeba histolytica and Entamoeba dispar
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6/8/09
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Description:Basic guidelines:
A. Multiple stool samples (at least 3) should be tested before a negative result is reported.
B. To maximize recovery of cysts, stool samples in formalin, or other fixatives, should be concentrated prior to microscopic examination (e.g.,10 min at 500 × g when using the formalin-ethyl- acetate concentration procedure). Exception: Specimens to be used for EIA or rapid cartridge assays should NOT be concentrated because antigens are lost during the procedure!
C. Choice of diagnostic techniques depends on available equipment and reagents, experience, and considerations of time and cost.
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Entamoeba_benchaid.pdf
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