Inactivated poliovirus type 2 vaccine delivered to rat skin via high density microprojection array elicits potent neutralising antibody responses

Details

• Alternative Title:
  Sci Rep
• Personal Author:
  Muller, David A. ; Pearson, Frances E. ; Fernando, Germain J.P. ; Agyei-Yeboah, Christiana ; Owens, Nick S. ; Corrie, Simon R. ; Crichton, Michael L. ; Wei, Jonathan C.J. ; Weldon, William C. ; Oberste, M. Steven ; Young, Paul R. ; Kendall, Mark A. F.
  More +
• Description:
  Polio eradication is progressing rapidly, and the live attenuated Sabin strains in the oral poliovirus vaccine (OPV) are being removed sequentially, starting with type 2 in April 2016. For risk mitigation, countries are introducing inactivated poliovirus vaccine (IPV) into routine vaccination programs. After April 2016, monovalent type 2 OPV will be available for type 2 outbreak control. Because the current IPV is not suitable for house-to-house vaccination campaigns (the intramuscular injections require health professionals), we developed a high-density microprojection array, the Nanopatch, delivered monovalent type 2 IPV (IPV2) vaccine to the skin. To assess the immunogenicity of the Nanopatch, we performed a dose-matched study in rats, comparing the immunogenicity of IPV2 delivered by intramuscular injection or Nanopatch immunisation. A single dose of 0.2 D-antigen units of IPV2 elicited protective levels of poliovirus antibodies in 100% of animals. However, animals receiving IPV2 by IM required at least 3 immunisations to reach the same neutralising antibody titres. This level of dose reduction (1/40th of a full dose) is unprecedented for poliovirus vaccine delivery. The ease of administration coupled with the dose reduction observed in this study points to the Nanopatch as a potential tool for facilitating inexpensive IPV for mass vaccination campaigns.
• Subjects:
  • Animals
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Antibody Formation
  • Female
  • Poliovirus
  • Poliovirus Vaccine, Inactivated
  • Rats
  • Rats, Wistar
  • Vaccination
• Source:
  Sci Rep. 2016; 6.
• Pubmed ID:
  26911254
• Pubmed Central ID:
  PMC4766532
• Document Type:
  Journal Article
• Volume:
  6
• Download URL:
  https://stacks.cdc.gov/view/cdc/38470/cdc_38470_DS1.pdf
• File Type:
  [PDF - 660.17 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7852720c61fd1229b87ef83becf38b9eb1ac7971fbe4c8d2034192a39c2aeaba