Evaluation of a Newly Developed Lateral Flow Immunoassay for the Diagnosis of Cryptococcosis

Details

• Alternative Title:
  Clin Infect Dis
• Personal Author:
  Lindsley, Mark D. ; Mekha, Nanthawan ; Baggett, Henry C. ; Surinthong, Yupha ; Autthateinchai, Rinrapas ; Sawatwong, Pongpun ; Harris, Julie R. ; Park, Benjamin J. ; Chiller, Tom ; Balajee, S. Arunmozhi ; Poonwan, Natteewan
• Description:
  Cryptococcosis is a common opportunistic infection of human immunodeficiency virus (HIV)-infected individuals mostly occurring in resource-limited countries. This study compares the performance of a recently developed lateral flow immunoassay (LFA) to blood culture and enzyme immunoassay (EIA) for the diagnosis of cryptococcosis.|Archived sera from 704 HIV-infected patients hospitalized for acute respiratory illness in Thailand were tested for cryptococcal antigenemia using EIA. All EIA-positive and a subset of EIA-negative sera were tested by LFA, with results recorded after 5 and 15 minutes incubation. Urine from patients with LFA- and EIA-positive sera was tested by LFA. Antigen results from patients with positive cryptococcal blood cultures were compared.|Of 704 sera, 92 (13%) were positive by EIA; among the 91 EIA-positive sera tested by LFA, 82 (90%) and 87 (96%) were LFA positive when read after 5 and 15 minutes, respectively. Kappa agreement of EIA and LFA for sera was 0.923 after 5 minutes and 0.959 after 15 minutes, respectively. Two of 373 EIA-negative sera were LFA positive at both time points. Of 74 urine specimens from EIA-positive patients, 52 (70.3%) were LFA positive. EIA was positive in 16 of 17 sera from blood culture-positive patients (94% sensitivity), and all sera were positive by LFA (100% sensitivity).|A high level of agreement was shown between LFA and EIA testing of serum. The LFA is a rapid, easy-to-perform assay that does not require refrigeration, demonstrating its potential usefulness as a point-of-care assay for diagnosis of cryptococcosis in resource-limited countries.
• Subjects:
  AIDS-Related Opportunistic Infections Antigens, Fungal Cryptococcosis HIV Infections Humans Immunoassay Sensitivity And Specificity
• Source:
  Clin Infect Dis. 2011; 53(4):321-325.
• Document Type:
  Journal Article
• Volume:
  53
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5687237ca4979adce19d9f87bf598baf4389a3ec80d3df9195707d5735b393bb
• Download URL:
  https://stacks.cdc.gov/view/cdc/3786/cdc_3786_DS1.pdf
• File Type:
  [PDF - 101.81 KB ]