Details:

Alternative Title:
J Virol Methods
Publisher's site:
http://dx.doi.org/10.1016/j.jviromet.2014.03.010
Personal Author:
Chang, Joy ; Omuomo, Kenneth ; Anyango, Emily ; Kingwara, Leonard ; Basiye, Frank ; ... More +
Chang, Joy ; Omuomo, Kenneth ; Anyango, Emily ; Kingwara, Leonard ; Basiye, Frank ; Morwabe, Alex ; Shanmugam, Vedapuri ; Nguyen, Shon ; Sabatier, Jennifer ; Zeh, Clement ; Ellenberger, Dennis Less -
Description:
Timely diagnosis and treatment of infants infected with HIV are critical for reducing infant mortality. High-throughput automated diagnostic tests like Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Qual Test (Roche CAPCTM Qual) and the Abbott Real Time HIV-1 Qualitative (Abbott Qualitative) can be used to rapidly expand early infant diagnosis testing services. In this study, the performance characteristics of the Abbott Qualitative were evaluated using two hundred dried blood spots (DBS) samples (100 HIV-1 positive and 100 HIV-1 negative) collected from infants attending the antenatal facilities in Kisumu, Kenya. The Abbott Qualitative results were compared to the diagnostic testing completed using the Roche CAPCTM Qual in Kenya. The sensitivity and specificity of the Abbott Qualitative were 99.0% (95% CI: 95.0-100.0) and 100.0% (95% CI: 96.0-100.0), respectively, and the overall reproducibility was 98.0% (95% CI: 86.0-100.0). The limits of detection for the Abbott Qualitative and Roche CAPCTM Qual were 56.5 and 6.9copies/mL at 95% CIs (p=0.005), respectively. The study findings demonstrate that the Abbott Qualitative test is a practical option for timely diagnosis of HIV in infants.
Subject:
[+]
Article
Automation, Laboratory
Blood
Desiccation
Early Diagnosis
HIV-1
HIV Infections
Humans
Infant
Reagent Kits, Diagnostic
Sensitivity And Specificity
Specimen Handling

Source:
J Virol Methods. 204:25-30.
Pubmed ID:
24726703
Pubmed Central ID:
PMC4720144
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Place as Subject:
Kenya
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:d8331c28ac9b0f9d0d4b68da34140387b92e44bea299cdb9908abf13233bd312
File Type:

Supporting Files

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