Details:

Alternative Title:
Vaccine
Personal Author:
Music, Nedzad ; Reber, Adrian J. ; Kim, Min-Chul ; York, Ian A. ; Kang, Sang-Moo
Music, Nedzad ; Reber, Adrian J. ; Kim, Min-Chul ; York, Ian A. ; Kang, Sang-Moo Less -
Description:
Current influenza vaccines induce strain-specific immunity to the highly variable hemagglutinin (HA) protein. It is therefore a high priority to develop vaccines that induce broadly cross-protective immunity to different strains of influenza. Since influenza A M2 proteins are highly conserved among different strains, five tandem repeats of the extracellular peptide of M2 in a membrane-anchored form on virus-like particles (VLPs) have been suggested to be a promising candidate for universal influenza vaccine. In this study, ferrets were intramuscularly immunized with 2009 H1N1 split HA vaccine ("Split") alone, influenza split vaccine supplemented with M2e5x VLP ("Split+M2e5x"), M2e5x VLP alone ("M2e5x"), or mock immunized. Vaccine efficacy was measured serologically and by protection against a serologically distinct viral challenge. Ferrets immunized with Split+M2e5x induced HA strain specific and conserved M2e immunity. Supplementation of M2e5x VLP to split vaccination significantly increased the immunogenicity of split vaccine compared to split alone. The Split+M2e5x ferret group showed evidence of cross-reactive protection, including faster recovery from weight loss, and reduced inflammation, as inferred from changes in peripheral leukocyte subsets, compared to mock-immunized animals. In addition, ferrets immunized with Split+M2e5x shed lower viral nasal-wash titers than the other groups. Ferrets immunized with M2e5x alone also show some protective effects, while those immunized with split vaccine alone induced no protective effects compared to mock-immunized ferrets. These studies suggest that supplementation of split vaccine with M2e5x-VLP may provide broader and improved cross-protection than split vaccine alone.
Subjects:
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Animals Antibodies, Viral Antibody Formation Article Cross-protection Cross Protection Ferret Ferrets Influenza A Virus, H1N1 Subtype Influenza Vaccines M2e Protein Male Orthomyxoviridae Infections T-Lymphocyte Subsets Vaccines, Virus-Like Particle Viral Matrix Proteins
Source:
Vaccine. 34(4):466-473.
Pubmed ID:
26709639
Pubmed Central ID:
PMC4713238
Document Type:
Journal Article
Funding:
R01 AI093772/AI/NIAID NIH HHS/United States ; CC999999/Intramural CDC HHS/United States ; AI119366/AI/NIAID NIH HHS/United States ; R21 AI119366/AI/NIAID NIH HHS/United States ; R01 AI105170/AI/NIAID NIH HHS/United States ; ... More +
R01 AI093772/AI/NIAID NIH HHS/United States ; CC999999/Intramural CDC HHS/United States ; AI119366/AI/NIAID NIH HHS/United States ; R21 AI119366/AI/NIAID NIH HHS/United States ; R01 AI105170/AI/NIAID NIH HHS/United States ; AI105170/AI/NIAID NIH HHS/United States ; AI093772/AI/NIAID NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:1224be95b5cbb60437ae57c913a15c6062b2f6e8b00a9400b89fb47d8ad8dce6
Download URL:
https://stacks.cdc.gov/view/cdc/37546/cdc_37546_DS1.pdf
File Type:

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