Details:

Alternative Title:
Nanotoxicology
Personal Author:
Porter, Dale W. ; Hubbs, Ann F. ; Chen, Bean T. ; McKinney, Walter ; Mercer, Robert R. ; ... More +
Porter, Dale W. ; Hubbs, Ann F. ; Chen, Bean T. ; McKinney, Walter ; Mercer, Robert R. ; Wolfarth, Michael G. ; Battelli, Lori ; Wu, Nianqiang ; Sriram, Krishnan ; Leonard, Stephen ; Andrew, Michael ; Willard, Patsy ; Tsuruoka, Shuji ; Endo, Morinobu ; Tsukada, Takayuki ; Munekane, Fuminori ; Frazer, David G. ; Castranova, Vincent Less -
Description:
This study investigated the in vivo pulmonary toxicity of inhaled multi-walled carbon nanotubes (MWCNT). Mice-inhaled aerosolized MWCNT (10 mg/m³, 5 h/day) for 2, 4, 8 or 12 days. MWCNT lung burden was linearly related to exposure duration. MWCNT-induced pulmonary inflammation was assessed by determining whole lung lavage (WLL) polymorphonuclear leukocytes (PMN). Lung cytotoxicity was assessed by WLL fluid LDH activities. WLL fluid albumin concentrations were determined as a marker of alveolar air-blood barrier integrity. These parameters significantly increased in MWCNT-exposed mice versus controls and were dose-dependent. Histopathologic alterations identified in the lung included (1) bronciolocentric inflammation, (2) bronchiolar epithelial hyperplasia and hypertrophy, (3) fibrosis, (4) vascular changes and (5) rare pleural penetration. MWCNT translocated to the lymph node where the deep paracortex was expanded after 8 or 12 days. Acute inhalation of MWCNT induced dose-dependent pulmonary inflammation and damage with rapid development of pulmonary fibrosis, and also demonstrated that MWCNT can reach the pleura after inhalation exposure.
Subjects:
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Aerosols Albumins Animals Article Bronchoalveolar Lavage Fluid Cell Survival Characterisation Cytokines Electron Spin Resonance Spectroscopy Fibrosis Inflammation Inhalation Exposure Lung Lung Burden Male Mice Mice, Inbred C57BL Microscopy, Electron, Transmission Nanotubes, Carbon Neutrophils Particle Size Pleural Penetration Pneumonia Surface Properties
Source:
Nanotoxicology. 7(7):1179-1194.
Pubmed ID:
22881873
Pubmed Central ID:
PMC4687396
Document Type:
Journal Article
Funding:
2927ZKKV/PHS HHS/United States ; CC999999/Intramural CDC HHS/United States
2927ZKKV/PHS HHS/United States ; CC999999/Intramural CDC HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:af8d0d7ecc9debbaa0ff48c96579d4e1669b3b1ad473b8aed9ba803df9fd4df8
Download URL:
https://stacks.cdc.gov/view/cdc/37009/cdc_37009_DS1.pdf
File Type:

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