Fusion with extracellular domain of cytotoxic T-lymphocyte-associated-antigen 4 leads to enhancement of immunogenicity of Hantaan virus DNA vaccines in C57BL/6 mice

Liu, Feng; Liang, Mifang; Cao, Shouchun; Liu, Qinzhi; Zhang, Quanfu; Li, Chuan; Zhang, Shuo; Wang, Shiwen; Li, Dexin

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Fusion with extracellular domain of cytotoxic T-lymphocyte-associated-antigen 4 leads to enhancement of immunogenicity of Hantaan virus DNA vaccines in C57BL/6 mice

Published Date:

Sep 23 2011

Source:

Virol J. 2011; 8:448.

[PDF-682.48 KB]

Details:

Alternative Title:

Virol J

Personal Author:

Liu, Feng ; Liang, Mifang ; Cao, Shouchun ; Liu, Qinzhi ; Zhang, Quanfu ; ... More ▼

Description:

Background

Hantaan virus (HTNV) is the causative agent of the most severe form of a rodent-borne disease known as hemorrhagic fever with renal syndrome (HFRS). A safe and effective HTNV vaccine is needed. Vaccination with DNA constructs expressing fused antigen with bioactive factors, has shown promising improvement of immunogenicity for viral agents in animal models, but the effect of fusion strategy on HTNV DNA vaccine has not been investigated.

Results

DNA plasmids encoding the HTNV nucleocapsid protein (N) and glycoprotein (Gn and Gc) in fusion to the extracellular domain of cytotoxic T-lymphocyte-associated-antigen 4 (eCTLA-4) targeting to antigen presenting cells (APCs) were constructed. Intramuscular immunization of mice with plasmids expressing eCTLA-4-HTNV-N/GP fusion proteins leads to a significant enhancement of the specific antibody response as well as cytotoxic T-lymphocyte (CTL) response in C57BL/6 mice. Moreover, this effect could be further augmented when co-administered with CpG motifs.

Conclusions

Modification of viral antigen in fusion to bioactive factor will be promising to confer efficient antigen presentation and improve the potency of DNA vaccine in mice.

Subject:

[+]

Document Type:

Journal Article

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

	license.txt	text/plain
	1743-422X-8-448-1.gif	image/gif
	1743-422X-8-448-1.jpg	image/jpeg
	1743-422X-8-448-2.gif	image/gif
	1743-422X-8-448-2.jpg	image/jpeg
	1743-422X-8-448-3.gif	image/gif
	1743-422X-8-448-3.jpg	image/jpeg
	1743-422X-8-448.nxml	text/plain

No Related Documents.

You May Also Like:

Vaccination with dengue virus-like particles induces humoral and cellular immune responses in mice

Personal Author:

Zhang, Shuo; Liang, Mifang; Gu, Wen; Li, Chuan; Miao, Fang; Wang, Xiaofang; Jin, Cong; Zhang, Li; Zhang, Fushun; Zhang, Quanfu; Jiang, Lifang; Li, Mengfeng; Li, Dexin;

Published Date:

Jun 30 2011

Source:

Virol J. 2011; 8:333.

Description:

BackgroundThe incidence of dengue, an infectious disease caused by dengue virus (DENV), has dramatically increased around the world in recent decades and is becoming a severe public health threat. However, there is currently no specific treatment for...

File Type:

[PDF - 602.00 KB]
Prospects for Universal Influenza Virus Vaccine

Personal Author:

Gerhard, Walter; Mozdzanowska, Krystyna; Zharikova, Darya;

Published Date:

Apr 2006

Source:

Emerg Infect Dis. 12(4):569-574.

Description:

The current vaccination strategy against influenza A and B viruses is vulnerable to the unanticipated emergence of epidemic strains that are poorly matched by the vaccine. A vaccine that is less sensitive to the antigenic evolution of the virus would...

File Type:

[PDF - 62.44 KB]
Immunogenicity in mice and rhesus monkeys vaccinated with recombinant vaccinia virus expressing bivalent E7E6 fusion proteins from human papillomavirus types 16 and 18

Personal Author:

Zhao, Li; Liu, Binlei; Ren, Jiao; Feng, Jing; Pang, Zheng; Gao, Jian; Zhang, Hui; Tan, Wenjie; Tian, Houwen; Ruan, Li;

Published Date:

Jun 15 2011

Source:

Virol J. 2011; 8:302.

Description:

BackgroundPersistent infection with high-risk human papillomavirus (HPV) is a predominant cause of cervical cancer, and HPV16 and HPV18 occur in 50% and 20% of cervical cancer cases, respectively. The viral oncogenes E6 and E7 are constitutively expr...

File Type:

[PDF - 1.11 MB]
DNA Vaccine Expressing Conserved Influenza Virus Proteins Protective Against H5N1 Challenge Infection in Mice

Personal Author:

Epstein, Suzanne L.; Tumpey, Terrence M.; Misplon, Julia A.; Lo, Chia-Yun; Cooper, Lynn A.; Subbarao, Kanta; Renshaw, Mary; Sambhara, Suryaprakash; Katz, Jacqueline M.;

Published Date:

Aug 2002

Source:

Emerg Infect Dis. 8(8):796-801.

Description:

Influenza vaccination practice, which is based on neutralizing antibodies, requires being able to predict which viral strains will be circulating. If an unexpected strain, as in the 1997 H5N1 Hong Kong outbreak, or even a pandemic emerges, appropriat...

File Type:

[PDF - 297.44 KB]
Nanoparticle Vaccines Encompassing the Respiratory Syncytial Virus (RSV) G Protein CX3C Chemokine Motif Induce Robust Immunity Protecting from Challenge and Disease

Personal Author:

Jorquera, Patricia A.; Choi, Youngjoo; Oakley, Katie E.; Powell, Thomas J.; Boyd, James G.; Palath, Naveen; Haynes, Lia M.; Anderson, Larry J.; Tripp, Ralph A.;

Published Date:

Sep 10 2013

Source:

PLoS One. 2013; 8(9).

Description:

Nanoparticle vaccines were produced using layer-by-layer fabrication and incorporating respiratory syncytial virus (RSV) G protein polypeptides comprising the CX3C chemokine motif. BALB/c mice immunized with G protein nanoparticle vaccines produced a...

File Type:

[PDF - 1.35 MB]