Fusion with extracellular domain of cytotoxic T-lymphocyte-associated-antigen 4 leads to enhancement of immunogenicity of Hantaan virus DNA vaccines in C57BL/6 mice

Details

• Alternative Title:
  Virol J
• Personal Author:
  Liu, Feng ; Liang, Mifang ; Cao, Shouchun ; Liu, Qinzhi ; Zhang, Quanfu ; Li, Chuan ; Zhang, Shuo ; Wang, Shiwen ; Li, Dexin
• Description:
  Background
  
  Hantaan virus (HTNV) is the causative agent of the most severe form of a rodent-borne disease known as hemorrhagic fever with renal syndrome (HFRS). A safe and effective HTNV vaccine is needed. Vaccination with DNA constructs expressing fused antigen with bioactive factors, has shown promising improvement of immunogenicity for viral agents in animal models, but the effect of fusion strategy on HTNV DNA vaccine has not been investigated.
  
  Results
  
  DNA plasmids encoding the HTNV nucleocapsid protein (N) and glycoprotein (Gn and Gc) in fusion to the extracellular domain of cytotoxic T-lymphocyte-associated-antigen 4 (eCTLA-4) targeting to antigen presenting cells (APCs) were constructed. Intramuscular immunization of mice with plasmids expressing eCTLA-4-HTNV-N/GP fusion proteins leads to a significant enhancement of the specific antibody response as well as cytotoxic T-lymphocyte (CTL) response in C57BL/6 mice. Moreover, this effect could be further augmented when co-administered with CpG motifs.
  
  Conclusions
  
  Modification of viral antigen in fusion to bioactive factor will be promising to confer efficient antigen presentation and improve the potency of DNA vaccine in mice.
  
  
• Subjects:
  Animals Antibodies, Viral Antigen-Presenting Cells Antigens, Viral Capsid Proteins Cell Line CpG Islands Cricetinae Female Flow Cytometry Hantaan Virus Hemorrhagic Fever With Renal Syndrome Humans Immunoconjugates Injections, Intramuscular Mice Mice, Inbred C57BL Plasmids Recombinant Fusion Proteins Signal Transduction T-Lymphocytes, Cytotoxic Vaccination Vaccines, DNA Viral Core Proteins

  More +
• Source:
  Virol J. 2011; 8:448.
• Document Type:
  Journal Article
• Volume:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:001a9b5429d39485ea407d6686c1eda1c45c6dc7884c44c8fd4c8246288b8ee9
• Download URL:
  https://stacks.cdc.gov/view/cdc/3646/cdc_3646_DS1.pdf
• File Type:
  [PDF - 682.48 KB ]