Details:

Alternative Title:
Virol J
Personal Author:
Liu, Feng ; Liang, Mifang ; Cao, Shouchun ; Liu, Qinzhi ; Zhang, Quanfu ; ... More +
Liu, Feng ; Liang, Mifang ; Cao, Shouchun ; Liu, Qinzhi ; Zhang, Quanfu ; Li, Chuan ; Zhang, Shuo ; Wang, Shiwen ; Li, Dexin Less -
Description:
Background

Hantaan virus (HTNV) is the causative agent of the most severe form of a rodent-borne disease known as hemorrhagic fever with renal syndrome (HFRS). A safe and effective HTNV vaccine is needed. Vaccination with DNA constructs expressing fused antigen with bioactive factors, has shown promising improvement of immunogenicity for viral agents in animal models, but the effect of fusion strategy on HTNV DNA vaccine has not been investigated.

Results

DNA plasmids encoding the HTNV nucleocapsid protein (N) and glycoprotein (Gn and Gc) in fusion to the extracellular domain of cytotoxic T-lymphocyte-associated-antigen 4 (eCTLA-4) targeting to antigen presenting cells (APCs) were constructed. Intramuscular immunization of mice with plasmids expressing eCTLA-4-HTNV-N/GP fusion proteins leads to a significant enhancement of the specific antibody response as well as cytotoxic T-lymphocyte (CTL) response in C57BL/6 mice. Moreover, this effect could be further augmented when co-administered with CpG motifs.

Conclusions

Modification of viral antigen in fusion to bioactive factor will be promising to confer efficient antigen presentation and improve the potency of DNA vaccine in mice.


Subjects:
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Animals Antibodies, Viral Antigen-Presenting Cells Antigens, Viral Capsid Proteins Cell Line CpG Islands Cricetinae Female Flow Cytometry Hantaan Virus Hemorrhagic Fever With Renal Syndrome Humans Immunoconjugates Injections, Intramuscular Mice Mice, Inbred C57BL Plasmids Recombinant Fusion Proteins Research Signal Transduction T-Lymphocytes, Cytotoxic Vaccination Vaccines, DNA Viral Core Proteins
Source:
Virol J. 2011; 8:448.
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:001a9b5429d39485ea407d6686c1eda1c45c6dc7884c44c8fd4c8246288b8ee9
Download URL:
https://stacks.cdc.gov/view/cdc/3646/cdc_3646_DS1.pdf
File Type:

Supporting Files

• 	license.txt	txt
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  	1743-422X-8-448.nxml	txt

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