Details:

Alternative Title:
Virol J
Personal Author:
Liu, Feng; Liang, Mifang; Cao, Shouchun; Liu, Qinzhi; Zhang, Quanfu; ... More +
Liu, Feng; Liang, Mifang; Cao, Shouchun; Liu, Qinzhi; Zhang, Quanfu; Li, Chuan; Zhang, Shuo; Wang, Shiwen; Li, Dexin; Less -
Description:
Background Hantaan virus (HTNV) is the causative agent of the most severe form of a rodent-borne disease known as hemorrhagic fever with renal syndrome (HFRS). A safe and effective HTNV vaccine is needed. Vaccination with DNA constructs expressing fused antigen with bioactive factors, has shown promising improvement of immunogenicity for viral agents in animal models, but the effect of fusion strategy on HTNV DNA vaccine has not been investigated. Results DNA plasmids encoding the HTNV nucleocapsid protein (N) and glycoprotein (Gn and Gc) in fusion to the extracellular domain of cytotoxic T-lymphocyte-associated-antigen 4 (eCTLA-4) targeting to antigen presenting cells (APCs) were constructed. Intramuscular immunization of mice with plasmids expressing eCTLA-4-HTNV-N/GP fusion proteins leads to a significant enhancement of the specific antibody response as well as cytotoxic T-lymphocyte (CTL) response in C57BL/6 mice. Moreover, this effect could be further augmented when co-administered with CpG motifs. Conclusions Modification of viral antigen in fusion to bioactive factor will be promising to confer efficient antigen presentation and improve the potency of DNA vaccine in mice.
Subject:
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Animals
Antibodies, Viral
Antigen-Presenting Cells
Antigens, Viral
Capsid Proteins
Cell Line
CpG Islands
Cricetinae
Female
Flow Cytometry
Hantaan Virus
Hemorrhagic Fever With Renal Syndrome
Humans
Immunoconjugates
Injections, Intramuscular
Mice
Mice, Inbred C57BL
Plasmids
Recombinant Fusion Proteins
Research
Signal Transduction
T-Lymphocytes, Cytotoxic
Vaccination
Vaccines, DNA
Viral Core Proteins

Source:
Virol J. 2011; 8:448.;
Document Type:
Journal Article;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:001a9b5429d39485ea407d6686c1eda1c45c6dc7884c44c8fd4c8246288b8ee9
File Type:

Supporting Files

• 	license.txt	txt
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