Details:

Alternative Title:
Contact Dermatitis
Publisher's site:
http://dx.doi.org/10.1111/cod.12169
Personal Author:
Siegel, Paul D. ; Fowler, Joseph F. ; Law, Brandon F. ; Warshaw, Erin M. ; Taylor, James S.
Siegel, Paul D. ; Fowler, Joseph F. ; Law, Brandon F. ; Warshaw, Erin M. ; Taylor, James S. Less -
Description:
Background

Epicutaneous patch tests are used to reproduce allergy and diagnose allergic contact dermatitis. Reliable allergen test preparations are required.

Objectives

The purpose of the present study was to measure the actual concentrations of nickel(II) sulfate hexahydrate (NiSO4), methyl methacrylate, formaldehyde, and glutaraldehyde, and to compare them with the labelled concentrations, in commercial patch test allergen preparations found in dermatology clinics where patch testing is routinely performed.

Materials and methods

The commercial in-date and out-of-date patch test allergen preparations concentrations of NiSO4, methyl methacrylate, formaldehyde and glutaraldehyde from one to three participating clinics were analysed with chromatographic or wet chemical techniques.

Results

NiSO4 and formaldehyde concentrations were at or above the labelled concentrations; however, formaldehyde loss occurred with storage. NiSO4 particulate was uniformly distributed throughout the petrolatum. ‘In-use’ methyl methacrylate reagent syringes all contained ≤ 56% of the 2% label concentration, with no observable relationship with expiration date. Lower methyl methacrylate cocentrations were consistently measured at the syringe tip end, suggesting loss resulting from methyl methacrylate’s volatility. The concentrations of glutaraldehyde patch test allergen preparations ranged from 27% to 45% of the labelled (1% in pet.) concentration, independently of expiration date.

Conclusions

Some false-negative methyl methacrylate, formaldehyde or glutaraldehyde patch test results may be attributable to instability of the test preparations.


Subject:
[+]
Allergens
Article
Concentration And Stability
Dermatitis, Allergic Contact
Drug Stability
Formaldehyde
Glutaral
Glutaraldehyde
Humans
Methyl Methacrylate
Methylmethacrylate
Nickel
Nickel Sulfate
Patch Test Allergens
Patch Tests
Formaldehyde

Source:
Contact Dermatitis. 70(5):309-315.
Pubmed ID:
24731086
Pubmed Central ID:
PMC4629838
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:2b109c03a8aec803751e1f8615c73422f8b131180556e00aa24ef824501630b7
File Type:

Supporting Files

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