Details:

Alternative Title:
Water (Basel)
Personal Author:
Kahler, Amy M. ; Johnson, Trisha B. ; Hahn, Donghyun ; Narayanan, Jothikumar ; Derado, Gordana ; ... More +
Kahler, Amy M. ; Johnson, Trisha B. ; Hahn, Donghyun ; Narayanan, Jothikumar ; Derado, Gordana ; Hill, Vincent R. Less -
Description:
In this study, hollow-fiber ultrafiltration (UF) was assessed for recovery of Escherichia coli, Clostridium perfringens spores, Cryptosporidium parvum oocysts, echovirus 1, and bacteriophages MS2 and ΦX174 from ground and surface waters. Microbes were seeded into twenty-two 50-L water samples that were collected from the Southeastern United States and concentrated to ∼500 mL by UF. Secondary concentration was performed for C. parvum by centrifugation followed by immunomagnetic separation. Secondary concentration for viruses was performed using centrifugal ultrafilters or polyethylene glycol precipitation. Nine water quality parameters were measured in each water sample to determine whether water quality data correlated with UF and secondary concentration recovery efficiencies. Average UF recovery efficiencies were 66%-95% for the six enteric microbes. Average recovery efficiencies for the secondary concentration methods were 35%-95% for C. parvum and the viruses. Overall, measured water quality parameters were not significantly associated with UF recovery efficiencies. However, recovery of ΦX174 was negatively correlated with turbidity. The recovery data demonstrate that UF can be an effective method for concentrating diverse microbes from ground and surface waters. This study highlights the utility of tangential-flow hollow fiber ultrafiltration for recovery of bacteria, viruses, and parasites from large volume environmental water samples.
Subjects:
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Article Centricon Plus-70 Groundwater Immunomagnetic Separation (IMS) Pathogen Detection Polyethylene Glycol Surface Water Ultrafiltration
Source:
Water (Basel). 7(3):1202-1216.
Pubmed ID:
26530003
Pubmed Central ID:
PMC4627901
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:b651d5b1e4d1dc466879210c58055e3ba60d13be22a36173db4d15ea80fbe172
Download URL:
https://stacks.cdc.gov/view/cdc/36356/cdc_36356_DS1.pdf
File Type:

Supporting Files

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