Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA

Chiang, Cheng-Feng; Lo, Michael K; Rota, Paul A; Spiropoulou, Christina F; Rollin, Pierre E

i

Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA

Supporting Files Public Domain

Jun 03 2010
By Chiang, Cheng-Feng ; Lo, Michael K ; Rota, Paul A ; ...

Details

Alternative Title:

Virol J
Personal Author:

Chiang, Cheng-Feng ; Lo, Michael K ; Rota, Paul A ; Spiropoulou, Christina F ; Rollin, Pierre E
Description:

Background

Outbreaks of Hendra (HeV) and Nipah (NiV) viruses have been reported starting in 1994 and 1998, respectively. Both viruses are capable of causing fatal disease in humans and effecting great economical loss in the livestock industry.

Results

Through screening of hybridomas derived from mice immunized with γ-irradiated Nipah virus, we identified two secreted antibodies; one reactive with the nucleocapsid (N) protein and the other, the phosphoprotein (P) of henipaviruses. Epitope mapping and protein sequence alignments between NiV and HeV suggest the last 14 amino acids of the carboxyl terminus of the N protein is the target of the anti-N antibody. The anti-P antibody recognizes an epitope in the amino-terminal half of P protein. These monoclonal antibodies were used to develop two antigen capture ELISAs, one for virus detection and the other for differentiation between NiV and HeV. The lower limit of detection of the capture assay with both monoclonal antibodies was 400 pfu. The anti-N antibody was used to successfully detect NiV in a lung tissue suspension from an infected pig.

Conclusion

The antigen capture ELISA developed is potentially affordable tool to provide rapid detection and differentiation between the henipaviruses.
Subjects:

Animals Antibodies, Monoclonal Antigens, Viral Cell Line Enzyme-Linked Immunosorbent Assay Epitope Mapping Hendra Virus Henipavirus Infections Humans Mice Mice, Inbred BALB C Nipah Virus Nucleocapsid Proteins Phosphoproteins Research Swine Swine Diseases
Source:

Virol J. 2010; 7:115.
Document Type:

Journal Article
Volume:

7
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:a705af21990eefe4c5f0a31601484b9db62cacc043b39b82223c96739df4ea65
Download URL:

https://stacks.cdc.gov/view/cdc/3631/cdc_3631_DS1.pdf
File Type:

[PDF - 1.04 MB ]

ON THIS PAGE

Details Supporting Files

CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.