Details:

Alternative Title:
Virol J
Personal Author:
Westerman, Larry E ; Jiang, Baoming ; McClure, Harold M ; Snipes-Magaldi, Lauren J ; Griffin, Dixie D ; ... More +
Westerman, Larry E ; Jiang, Baoming ; McClure, Harold M ; Snipes-Magaldi, Lauren J ; Griffin, Dixie D ; Shin, Gary ; Gentsch, Jon R ; Glass, Roger I Less -
Description:
Background

To effectively analyze the requirements for protection to rotavirus infection, a reliable animal model that reasonably mimics infection and disease in humans is needed. A requirement for an effective animal model is the availability of appropriate rotavirus stocks for challenge.

Results

A new simian rotavirus, designated YK-1, was isolated from a 2-year-old immunodeficient pigtailed macaque with chronic diarrhea. YK-1 was distinguishable by electropherotype from the other simian rotavirus strains, SA11 and RRV. One variant of YK-1, clone 311, which was isolated after adaptation and plaque purification in cell cultures, displayed an unusual RNA electropherotype with an abnormally migrating gene 11 segment. Sequence analysis demonstrated a genetic rearrangement that involved a partial duplication of the gene 11 ORF encoding NSP5. YK-1 was identified as a Group A rotavirus belonging to subgroup 1. To further characterize the YK-1 strain, the genes encoding VP4, VP7, and NSP4 were sequenced. Analysis of VP4 and VP7 gene fragments suggests that this strain is a G3P[3] rotavirus and is closely related to the simian rotavirus strain RRV. Serotype analysis also identified YK-1 as a G3 rotavirus. The NSP4 genotype of YK-1 is C, the same genotype as RRV.

Conclusion

This newly isolated rotavirus, YK-1, is being used to establish a nonhuman primate model for studying the infectivity, immunity, and pathogenesis of rotavirus and for evaluating candidate rotavirus vaccines.


Subject:
[+]
Animals
Antigens, Viral
Capsid Proteins
Diarrhea
Feces
Glycoproteins
Humans
Macaca Nemestrina
Molecular Sequence Data
Monkey Diseases
Research
RNA, Viral
Rotavirus
Rotavirus Infections
Sequence Analysis, DNA
Serotyping
Toxins, Biological
Viral Nonstructural Proteins

Source:
Virol J. 2006; 3:40.
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:221ed3d46c9c9d6ceba7d39b0ff0ac00dbca521fa80b1d0fa0ae7b7f562c7bf0
File Type:

Supporting Files

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