Isolation and characterization of a new simian rotavirus, YK-1

Details

• Alternative Title:
  Virol J
• Personal Author:
  Westerman, Larry E ; Jiang, Baoming ; McClure, Harold M ; Snipes-Magaldi, Lauren J ; Griffin, Dixie D ; Shin, Gary ; Gentsch, Jon R ; Glass, Roger I
• Description:
  Background
  
  To effectively analyze the requirements for protection to rotavirus infection, a reliable animal model that reasonably mimics infection and disease in humans is needed. A requirement for an effective animal model is the availability of appropriate rotavirus stocks for challenge.
  
  Results
  
  A new simian rotavirus, designated YK-1, was isolated from a 2-year-old immunodeficient pigtailed macaque with chronic diarrhea. YK-1 was distinguishable by electropherotype from the other simian rotavirus strains, SA11 and RRV. One variant of YK-1, clone 311, which was isolated after adaptation and plaque purification in cell cultures, displayed an unusual RNA electropherotype with an abnormally migrating gene 11 segment. Sequence analysis demonstrated a genetic rearrangement that involved a partial duplication of the gene 11 ORF encoding NSP5. YK-1 was identified as a Group A rotavirus belonging to subgroup 1. To further characterize the YK-1 strain, the genes encoding VP4, VP7, and NSP4 were sequenced. Analysis of VP4 and VP7 gene fragments suggests that this strain is a G3P[3] rotavirus and is closely related to the simian rotavirus strain RRV. Serotype analysis also identified YK-1 as a G3 rotavirus. The NSP4 genotype of YK-1 is C, the same genotype as RRV.
  
  Conclusion
  
  This newly isolated rotavirus, YK-1, is being used to establish a nonhuman primate model for studying the infectivity, immunity, and pathogenesis of rotavirus and for evaluating candidate rotavirus vaccines.
  
  
• Subjects:
  Animals Antigens, Viral Capsid Proteins Diarrhea Feces Glycoproteins Humans Macaca Nemestrina Molecular Sequence Data Monkey Diseases Research RNA, Viral Rotavirus Rotavirus Infections Sequence Analysis, DNA Serotyping Toxins, Biological Viral Nonstructural Proteins

  More +
• Source:
  Virol J. 2006; 3:40.
• Document Type:
  Journal Article
• Volume:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:c955f9c9b8479df1bbee1ae54bd55316158861d6c89d221424d2ce61a3a9b479e95a652881ed01cfa55d36fc05a7dfcec4f56d01382d8133d18dfc11daeb1a49
• Download URL:
  https://stacks.cdc.gov/view/cdc/3622/cdc_3622_DS1.pdf
• File Type:
  [PDF - 353.46 KB ]