Details:

Alternative Title:
PLoS Negl Trop Dis
Publisher's site:
http://dx.doi.org/10.1371/journal.pntd.0004168
Personal Author:
Anderson, John P. ; Rascoe, Lisa N. ; Levert, Keith ; Chastain, Holly M. ; Reed, Matthew S. ; ... More +
Anderson, John P. ; Rascoe, Lisa N. ; Levert, Keith ; Chastain, Holly M. ; Reed, Matthew S. ; Rivera, Hilda N. ; McAuliffe, Isabel ; Zhan, Bin ; Wiegand, Ryan E. ; Hotez, Peter J. ; Wilkins, Patricia P. ; Pohl, Jan ; Handali, Sukwan Less -
Description:
The clinical spectrum of human disease caused by the roundworms Toxocara canis and Toxocara cati ranges from visceral and ocular larva migrans to covert toxocariasis. The parasite is not typically recovered in affected tissues, so detection of parasite-specific antibodies is usually necessary for establishing a diagnosis. The most reliable immunodiagnostic methods use the Toxocara excretory-secretory antigens (TES-Ag) in ELISA formats to detect Toxocara-specific antibodies. To eliminate the need for native parasite materials, we identified and purified immunodiagnostic antigens using 2D gel electrophoresis followed by electrospray ionization mass spectrometry. Three predominant immunoreactive proteins were found in the TES; all three had been previously described in the literature: Tc-CTL-1, Tc-TES-26, and Tc-MUC-3. We generated Escherichia coli expressed recombinant proteins for evaluation in Luminex based immunoassays. We were unable to produce a functional assay with the Tc-MUC-3 recombinant protein. Tc-CTL-1 and Tc-TES-26 were successfully coupled and tested using defined serum batteries. The use of both proteins together generated better results than if the proteins were used individually. The sensitivity and specificity of the assay for detecting visceral larval migrans using Tc-CTL-1 plus Tc-TES-26 was 99% and 94%, respectively; the sensitivity for detecting ocular larval migrans was 64%. The combined performance of the new assay was superior to the currently available EIA and could potentially be employed to replace current assays that rely on native TES-Ag.
Subjects:
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Research Article
Source:
PLoS Negl Trop Dis. 9(10).
Pubmed ID:
26485145
Pubmed Central ID:
PMC4618131
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:04940446cf476880fd07217715812eb8748e34d15cb6b9666b01956da2ebe3ac
Download URL:
https://stacks.cdc.gov/view/cdc/35510/cdc_35510_DS1.pdf
File Type:

Supporting Files

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