Performance of Cepheid® Xpert MTB/RIF® and TB Biochip® MDR in two regions of Russia with a high prevalence of drug-resistant tuberculosis

Kurbatova, Ekaterina V.; Kaminski, Dorothy A.; Erokhin, Vladislav V.; Volchenkov, Grigory V.; Andreevskaya, Sofia N.; Chernousova, Larisa N.; Demikhova, Olga V.; Ershova, Julia V.; Kaunetis, Natalia V.; Kuznetsova, Tatiana A.; Larionova, Elena E.; Smirnova, Tatiana G.; Somova, Tatiana R.; Vasilieva, Irina A.; Vorobieva, Alena V.; Zolkina, Svetlana S.; Cegielski, J. Peter

doi:10.1007/s10096-012-1798-0

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Performance of Cepheid® Xpert MTB/RIF® and TB Biochip® MDR in two regions of Russia with a high prevalence of drug-resistant tuberculosis

6 2013
By Kurbatova, Ekaterina V. ; Kaminski, Dorothy A. ; Erokhin, Vladislav V. ; ...
http://dx.doi.org/10.1007/s10096-012-1798-0
Source: Eur J Clin Microbiol Infect Dis. 32(6):735-743

[PDF-511.98 KB]

English

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Details:

Alternative Title:

Eur J Clin Microbiol Infect Dis
Personal Author:

Kurbatova, Ekaterina V. ; Kaminski, Dorothy A. ; Erokhin, Vladislav V. ; Volchenkov, Grigory V. ; Andreevskaya, Sofia N. ; ... More +

Kurbatova, Ekaterina V. ; Kaminski, Dorothy A. ; Erokhin, Vladislav V. ; Volchenkov, Grigory V. ; Andreevskaya, Sofia N. ; Chernousova, Larisa N. ; Demikhova, Olga V. ; Ershova, Julia V. ; Kaunetis, Natalia V. ; Kuznetsova, Tatiana A. ; Larionova, Elena E. ; Smirnova, Tatiana G. ; Somova, Tatiana R. ; Vasilieva, Irina A. ; Vorobieva, Alena V. ; Zolkina, Svetlana S. ; Cegielski, J. Peter Less -
Description:

Purpose

To assess the performance of Cepheid® Xpert MTB/RIF® (“Xpert”) and TB Biochip® MDR (“TB-Biochip”).

Methods

Sputum specimens from adults with presumptive tuberculosis (TB) were homogenized and split for (1) direct Xpert and microscopy, and (2) concentration for Xpert, microscopy, culture (Lowenstein-Jensen [LJ] solid media and Mycobacteria Growth Indicator Tube® [MGIT]), indirect drug susceptibility testing (DST) using the absolute concentration method and MGIT, and TB-Biochip.

Results

In total, 109 of 238 (45.8%) specimens were culture-positive for M. tuberculosis complex (MTBC), and of these, 67 isolates were rifampicin resistant (RIF-R) by phenotypic DST, and 64/67 (95.5%) were isoniazid resistant (INH-R). Compared to culture of the same specimen, a single direct Xpert was more sensitive for detecting MTBC (95.3%, 95% confidence interval [CI], 90.0–98.3%) than direct (59.6%, 95% CI, 50.2–68.5%) or concentrated smear (85.3%, 95% CI, 77.7–91.1%) or LJ culture (80.8%, 95% CI, 72.4–87.5%); specificity was 86.0% (95% CI 78.9–91.3%). Compared with MGIT DST, Xpert correctly identified 98.2% (95% CI, 91.5–99.9%) of RIF-R and 95.5% (95% CI, 85.8–99.2%) of RIF-susceptible (RIF-S) specimens. In a subset of 104 specimens, the sensitivity of TB-Biochip for MTBC detection compared to culture was 97.3% (95% CI, 91.0–99.5%); specificity was 78.1% (95% CI, 61.5– 89.9%). TB-Biochip correctly identified 100% (95% CI, 94.2–100%) of RIF-R, 94.7% (95% CI, 76.7–99.7%) of RIF-S, 98.2% (95% CI, 91.4–99.9%) of INH-R, and 78.6% (95% CI, 52.1–94.2%) of INH-S specimens, compared to MGIT DST.

Conclusions

Xpert and Biochip were similar in accuracy for detecting MTBC and RIF resistance compared to conventional culture methods.
Keywords:

[+]

Adult Bacteriological Techniques Cepheid® Xpert MTB/RIF® Drug Resistance Humans Microbial Sensitivity Tests Mycobacterium Tuberculosis Prevalence Rapid Diagnosis Reproducibility Of Results Russia Sensitivity And Specificity Sputum Tuberculosis Tuberculosis, Multidrug-Resistant
Source:

Eur J Clin Microbiol Infect Dis. 32(6):735-743
Pubmed ID:

23263819
Pubmed Central ID:

PMC4596226
Document Type:

Journal Article
Funding:

CC999999/ImCDC/Intramural CDC HHSUnited States/
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:40aa3dfe2ce9103778a42bd87995ffcc79b37b7b1cb52cda8bcf79d617faf686
Download URL:

https://stacks.cdc.gov/view/cdc/34839/cdc_34839_DS1.pdf
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