Immunogenicity and efficacy of chimeric dengue vaccine (DENVax) formulations in interferon-deficient AG129 mice

Brewoo, Joseph N.; Kinney, Richard M.; Powell, Tim D.; Arguello, John J.; Silengo, Shawn J.; Partidos, Charalambos D.; Huang, Claire Y.-H.; Stinchcomb, Dan T.; Osorio, Jorge E.

doi:10.1016/j.vaccine.2011.11.072

Advanced Search

Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

Advanced Search
Custom Query

All these words:

For very narrow results

This exact word or phrase:

When looking for a specific result

Any of these words:

Best used for discovery & interchangable words

None of these words:

Recommended to be used in conjunction with other fields

Language:

Dates

Publication Date Range:

to

Document Data

Title:

Document Type:

Library

Collection:

Series:

People

Author:

Clear All

Query Builder

Query box

Clear All

For additional assistance using the Custom Query please check out our Help Page

CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners. As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.

i

Immunogenicity and efficacy of chimeric dengue vaccine (DENVax) formulations in interferon-deficient AG129 mice

2 14 2012
By Brewoo, Joseph N. ; Kinney, Richard M. ; Powell, Tim D. ; ...
http://dx.doi.org/10.1016/j.vaccine.2011.11.072
Source: Vaccine. 30(8):1513-1520

[PDF-734.56 KB]

English

Details Supporting Files You May Also Like

Details:

Alternative Title:

Vaccine
Personal Author:

Brewoo, Joseph N. ; Kinney, Richard M. ; Powell, Tim D. ; Arguello, John J. ; Silengo, Shawn J. ; ... More +

Brewoo, Joseph N. ; Kinney, Richard M. ; Powell, Tim D. ; Arguello, John J. ; Silengo, Shawn J. ; Partidos, Charalambos D. ; Huang, Claire Y.-H. ; Stinchcomb, Dan T. ; Osorio, Jorge E. Less -
Description:

Formulations of chimeric dengue vaccine (DENVax) viruses containing the pre-membrane (prM) and envelope (E) genes of serotypes 1-4 expressed in the context of the attenuated DENV-2 PDK-53 genome were tested for safety, immunogenicity and efficacy in interferon receptor knock-out mice (AG129). Monovalent formulations were safe and elicited robust neutralizing antibody responses to the homologous virus and only limited cross-reactivity to other serotypes. A single dose of monovalent DENVax-1, -2, or -3 vaccine provided eighty or greater percent protection against both wild-type (wt) DENV-1 (Mochizuki strain) and DENV-2 (New Guinea C strain) challenge viruses. A single dose of monovalent DENVax-4 also provided complete protection against wt DENV-1 challenge and significantly increased the survival times after challenge with wt DENV-2. In studies using tetravalent mixtures, DENVax ratios were identified that: (i) caused limited viremia, (ii) induced serotype-specific neutralizing antibodies to all four DENV serotypes with different hierarchies, and (iii) conferred full protection against clinical signs of disease following challenge with either wt DENV-1 or DENV-2 viruses. Overall, these data highlight the immunogenic profile of DENVax, a novel candidate tetravalent dengue vaccine and the advantage of sharing a common attenuated genomic backbone among the DENVax monovalent vaccines that confer protection against homologous or heterologous virus challenge.
Subjects:

[+]

Animals Antibodies, Neutralizing Antibodies, Viral Body Temperature Body Weight Dengue Dengue Vaccines Disease Models, Animal Interferons Mice Mice, Knockout Survival Analysis Vaccines, Attenuated Vaccines, Synthetic Viral Load Viremia
Keywords:

[+]

AG129 Mice Dengue 2 PDK-53 Chimeras Dengue Vaccines DENVax
Source:

Vaccine. 30(8):1513-1520
Pubmed ID:

22178727
Pubmed Central ID:

PMC4592107
Document Type:

Journal Article
Funding:

U01 AI070443-04/AI/NIAID NIH HHSUnited States/ ; U01 AI070443-01/AI/NIAID NIH HHSUnited States/ ; 5-U01-AI070443/AI/NIAID NIH HHSUnited States/ ; CC999999/ImCDC/Intramural CDC HHSUnited States/ ; U01 AI070443/AI/NIAID NIH HHSUnited States/ ; ... More +

U01 AI070443-04/AI/NIAID NIH HHSUnited States/ ; U01 AI070443-01/AI/NIAID NIH HHSUnited States/ ; 5-U01-AI070443/AI/NIAID NIH HHSUnited States/ ; CC999999/ImCDC/Intramural CDC HHSUnited States/ ; U01 AI070443/AI/NIAID NIH HHSUnited States/ ; U01 AI070443-03/AI/NIAID NIH HHSUnited States/ ; U01 AI070443-02/AI/NIAID NIH HHSUnited States/ Less -
Volume:

30
Issue:

8
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha-512:ad0ac7c9e7af13e663c4a2ce6d93384efec6ded578f737243eeacd1acffd93e31ae00462fbb88b90c680d87881b044246b10a1576362cb0796b793a2843b411d
Download URL:

https://stacks.cdc.gov/view/cdc/34674/cdc_34674_DS1.pdf
File Type:

	nihms724191f4.jpg	jpeg
	nihms724191.nxml	xml
	nihms724191f1.gif	gif
	nihms724191f1.jpg	jpeg
	nihms724191f2.gif	gif
	nihms724191f2.jpg	jpeg
	nihms724191f3.gif	gif
	nihms724191f3.jpg	jpeg
	nihms724191f4.gif	gif

More +