Details:

Alternative Title:
Virology
Publisher's site:
http://dx.doi.org/10.1016/j.virol.2013.08.031
Personal Author:
Othumpangat, Sreekumar ; Noti, John D. ; Blachere, Francoise M. ; Beezhold, Donald H.
Othumpangat, Sreekumar ; Noti, John D. ; Blachere, Francoise M. ; Beezhold, Donald H. Less -
Description:
Understanding the host response to influenza A virus infection is essential for developing intervention approaches. We show that infection of human alveolar epithelial cells and human bronchial epithelial cells with influenza A for 3h resulted in down-regulation of host hsa-miRNA-548an (miRNA-548an) which triggered the overexpression of influenza non-structural-1A binding protein (IVNS1ABP, herein referred to as NS1ABP). Reduced NS1ABP mRNA and NS1ABP protein expression after transfection of miRNA-548an mimic or increased NS1ABP mRNA and NS1ABP protein expression after transfection of miRNA-548an inhibitor provided evidence that miRNA-548an is involved in the regulation of NS1ABP. Transfection of cells with inhibitor led to reduced apoptosis of infected cells while transfection of mimic led to increased apoptosis and reduced influenza copy number suggesting that NS1ABP has a role in viral maintenance. Thus, miRNA-548an may be an important target in controlling the early stage infection of influenza A.
Subject:
[+]
Apoptosis
Article
Cell Line
Epithelial Cells
Gene Expression Regulation
Humans
Influenza A Virus
Influenza Virus
Lung Epithelial Cells
MicroRNAs
MiRNA
NS1A Binding Protein
Nuclear Proteins
Transcription Factors

Source:
Virology. 447(0):84-94.
Pubmed ID:
24210102
Pubmed Central ID:
PMC4591961
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:5bd50dd8fea96fe2dad741eeeceeebe1a16e068193c70c7267cb8781af1fa413
File Type:

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