Nucleic Acid Testing by Public Health Referral Laboratories for Public Health Laboratories Using the U.S. HIV Diagnostic Testing Algorithm

Details

• Alternative Title:
  J Clin Virol
• Personal Author:
  Wesolowski, Laura G. ; Wroblewski, Kelly ; Bennett, Spencer B. ; Parker, Monica M. ; Hagan, Celia ; Ethridge, Steven F. ; Rhodes, Jeselyn ; Sullivan, Timothy J. ; Ignacio-Hernando, Imelda ; Werner, Barbara G. ; Owen, S. Michele
• Description:
  Background
  
  Many public health laboratories adopting the U.S. HIV laboratory testing algorithm do not have a nucleic acid test (NAT), which is needed when the third-or fourth-generation HIV screening immunoassay is reactive and the antibody-based supplemental test is non-reactive or indeterminate.
  
  Objectives
  
  Among public health laboratories utilizing public health referral laboratories for NAT conducted as part of the algorithm, we evaluated the percentage of screening immunoassays needing NAT, the number of specimens not meeting APTIMA (NAT) specifications, time to APTIMA result, the proportion of acute infections (i.e., reactive APTIMA) among total infections, and screening immunoassay specificity.
  
  Study Design
  
  From August 2012 to April 2013, 22 laboratories enrolled to receive free APTIMA (NAT) at New York or Florida public health referral laboratories. Data were analyzed for testing conducted until June 2013.
  
  Conclusions
  
  Public health referral laboratories provide a feasible option for conducting NAT. Reducing the time from specimen collection to submission of specimens for NAT is an important step toward maximizing the public health impact of identifying acute infections.
  
  
• Subjects:
  AIDS Serodiagnosis Algorithms Article HIV-1 HIV-2 HIV Infections Humans Immunoassay Laboratories Mass Screening Nucleic Acid Amplification Techniques Referral And Consultation RNA, Viral Sensitivity And Specificity

  More +
• Source:
  J Clin Virol. 65:6-10.
• Pubmed ID:
  25766979
• Pubmed Central ID:
  PMC4586070
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States ; U60HM000803/HM/NCHM CDC HHS/United States
• Name as Subject:
  Centers for Disease Control and Prevention (U.S.)
• Place as Subject:
  Florida ; New York
• Volume:
  65
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:06035191af96c39ba6a5be21ff8f81ba3c9ff4acc4b64b0ba27d622e9cab0205
• Download URL:
  https://stacks.cdc.gov/view/cdc/34537/cdc_34537_DS1.pdf
• File Type:
  [PDF - 144.61 KB ]