Details:

Alternative Title:
J Med Entomol
Personal Author:
Brault, Aaron C. ; Fang, Ying ; Reisen, William K.
Brault, Aaron C. ; Fang, Ying ; Reisen, William K. Less -
Description:
Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex TaqMan or real-time semiquantitative reverse transcription polymerase chain reaction (RT-PCR) assay in which three virus specific primer-probe sets were used in the same reaction is described herein for the detection of western equine encephalomyelitis, St. Louis encephalitis and West Nile viral RNA. Laboratory validation and field data from 10 transmission seasons are reported. The comparative sensitivity and specificity of this multiplex assay to singleplex RT-PCR as well as an antigen detection (rapid analyte measurement platform) and standard plaque assays indicate this assay to be rapid and useful in providing mosquito infection data to estimate outbreak risk.
Subjects:
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Animals Article California Culicidae Encephalitis, St. Louis Encephalitis Virus, St. Louis Encephalitis Virus, Western Equine Encephalomyelitis, Western Equine Mosquito Multiplex Multiplex Polymerase Chain Reaction RNA, Viral Sensitivity And Specificity St. Louis Encephalitis Virus Surveillance Western Equine Encephalitis Virus West Nile Fever West Nile Virus
Source:
J Med Entomol. 52(3):491-499
Pubmed ID:
26334826
Pubmed Central ID:
PMC4581483
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHSUnited States/ ; R01 AI055607/AI/NIAID NIH HHSUnited States/ ; R01AI-055607/AI/NIAID NIH HHSUnited States/
CC999999/Intramural CDC HHSUnited States/ ; R01 AI055607/AI/NIAID NIH HHSUnited States/ ; R01AI-055607/AI/NIAID NIH HHSUnited States/ Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:580f0a58f82c2312ebc18318bf2ad60b36808bb17d6643f4cffa42d69717b33f
Download URL:
https://stacks.cdc.gov/view/cdc/34510/cdc_34510_DS1.pdf
File Type:

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