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COMPARISON OF BIOCHEMICAL AND MOLECULAR TESTS FOR DETECTING INSECTICIDE RESISTANCE DUE TO INSENSITIVE ACETYLCHOLINESTERASE IN CULEX QUINQUEFASCIATUS1
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Dec 2012
Source: J Am Mosq Control Assoc. 28(4):323-326. -
Alternative Title:J Am Mosq Control Assoc
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Description:Insecticide resistance to organophosphates and carbamates can be the result of changes in acetylcholinesterase activity conferred by the ACE-1 mutation. Detection of this altered target site mutation is important in guiding informed decisions for resistance management. In this study we compared a competitive enzyme assay with a polymerase chain reaction assay utilizing a restriction enzyme. Both assays detected the ACE-1 mutation in Culex quinquefasciatus and agreement was 100%. The costs and benefits of each assay are presented.
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Pubmed ID:23393758
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Pubmed Central ID:PMC4562403
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