COMPARISON OF BIOCHEMICAL AND MOLECULAR TESTS FOR DETECTING INSECTICIDE RESISTANCE DUE TO INSENSITIVE ACETYLCHOLINESTERASE IN CULEX QUINQUEFASCIATUS1

Details

• Alternative Title:
  J Am Mosq Control Assoc
• Personal Author:
  SCOTT, MARIAH L. ; McALLISTER, JANET C.
• Description:
  Insecticide resistance to organophosphates and carbamates can be the result of changes in acetylcholinesterase activity conferred by the ACE-1 mutation. Detection of this altered target site mutation is important in guiding informed decisions for resistance management. In this study we compared a competitive enzyme assay with a polymerase chain reaction assay utilizing a restriction enzyme. Both assays detected the ACE-1 mutation in Culex quinquefasciatus and agreement was 100%. The costs and benefits of each assay are presented.
• Subjects:
  Acetylcholinesterase Animals Cholinesterase Inhibitors Culex Gene Expression Regulation, Enzymologic Insecticide Resistance Insecticides
• Source:
  J Am Mosq Control Assoc. 28(4):323-326.
• Pubmed ID:
  23393758
• Pubmed Central ID:
  PMC4562403
• Document Type:
  Journal Article
• Funding:
  JVM6/Intramural CDC HHS/United States
• Volume:
  28
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f1e64f010c50d1fa5f28a177bdd247f83f1f3213257bc52556aeebf1bb540e3b
• Download URL:
  https://stacks.cdc.gov/view/cdc/34135/cdc_34135_DS1.pdf
• File Type:
  [PDF - 242.95 KB ]