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PERFORMANCE OF SUCCINYLACETONE ASSAYS AND THEIR ASSOCIATED PROFICIENCY TESTING OUTCOMES

Supporting Files
File Language:
English


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  • Alternative Title:
    Clin Biochem
  • Personal Author:
    Adam, BW ; Hall, EM ; Meredith, NK ; Lim, TH ; Haynes, CA ; Hannon, WH ; De Jesus, VR
  • Description:
    Background ; Succinylacetone (SUAC) is the primary metabolic marker for hepatorenal tyrosinemia. ; Materials and Methods ; We used results reported for dried-blood-spot proficiency testing (PT) specimens and hepatorenal tyrosinemia patients’ newborn screening (NBS) samples to demonstrate analytic biases in SUAC recoveries and differences in presumptive clinical classifications. ; Results ; SUAC recoveries from non-kit and NeoBase™ kit tandem mass spectrometry methods were markedly different. Kit users that set high cutoff values submitted discordant clinical assessments of “within normal limits” for PT specimens enriched with 10–15 µmol SUAC/L blood. SUAC levels in tyrosinemia patients’ NBS samples analyzed by NeoBase™ kit were lower than those in samples analyzed by non-kit methods. ; Conclusions ; Analytic biases in SUAC recoveries were consistent from 2009–2011. Discordant clinical assessments of PT specimens were associated with high cutoff values for NeoBase™ kit results. Method-related differences in SUAC concentrations of tyrosinemia patients’ samples were consistent with those of PT specimens.
  • Subjects:
  • Source:
    Clin Biochem. 45(18):1658-1663.
  • Pubmed ID:
    22906829
  • Pubmed Central ID:
    PMC4557813
  • Document Type:
    Journal Article
  • Funding:
    CC999999/Intramural CDC HHS/United States
  • Volume:
    45
  • Issue:
    18
  • Download URL:
    https://stacks.cdc.gov/view/cdc/34051/cdc_34051_DS1.pdf
  • File Type:
    Filetype[PDF - 1.07 MB]
  • Collection(s):
    CDC Public Access
  • Main Document Checksum:
    urn:sha256:f5241b9bb57d7bd4f2f05c9b11a1189480c7eabdf4d0557dc1441ad24c5fbb09
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