PERFORMANCE OF SUCCINYLACETONE ASSAYS AND THEIR ASSOCIATED PROFICIENCY TESTING OUTCOMES

Adam, BW; Hall, EM; Meredith, NK; Lim, TH; Haynes, CA; Hannon, WH; De Jesus, VR

doi:10.1016/j.clinbiochem.2012.08.007

i

PERFORMANCE OF SUCCINYLACETONE ASSAYS AND THEIR ASSOCIATED PROFICIENCY TESTING OUTCOMES

Supporting Files

Aug 11 2012
By Adam, BW ; Hall, EM ; Meredith, NK ; ...

File Language:

English

Details

Alternative Title:

Clin Biochem
Personal Author:

Adam, BW ; Hall, EM ; Meredith, NK ; Lim, TH ; Haynes, CA ; Hannon, WH ; De Jesus, VR
Description:

Background

Succinylacetone (SUAC) is the primary metabolic marker for hepatorenal tyrosinemia.

Materials and Methods

We used results reported for dried-blood-spot proficiency testing (PT) specimens and hepatorenal tyrosinemia patients’ newborn screening (NBS) samples to demonstrate analytic biases in SUAC recoveries and differences in presumptive clinical classifications.

Results

SUAC recoveries from non-kit and NeoBase™ kit tandem mass spectrometry methods were markedly different. Kit users that set high cutoff values submitted discordant clinical assessments of “within normal limits” for PT specimens enriched with 10–15 µmol SUAC/L blood. SUAC levels in tyrosinemia patients’ NBS samples analyzed by NeoBase™ kit were lower than those in samples analyzed by non-kit methods.

Conclusions

Analytic biases in SUAC recoveries were consistent from 2009–2011. Discordant clinical assessments of PT specimens were associated with high cutoff values for NeoBase™ kit results. Method-related differences in SUAC concentrations of tyrosinemia patients’ samples were consistent with those of PT specimens.
Subjects:

Analytic Bias Biological Assay Dried Blood Spot Testing Heptanoates Humans Infant, Newborn Laboratory Proficiency Testing Linear Models Neonatal Screening Tandem Mass Spectrometry
Source:

Clin Biochem. 45(18):1658-1663.
Pubmed ID:

22906829
Pubmed Central ID:

PMC4557813
Document Type:

Journal Article
Funding:

CC999999/Intramural CDC HHS/United States
Volume:

45
Issue:

18
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:f5241b9bb57d7bd4f2f05c9b11a1189480c7eabdf4d0557dc1441ad24c5fbb09
Download URL:

https://stacks.cdc.gov/view/cdc/34051/cdc_34051_DS1.pdf
File Type:

[PDF - 1.07 MB ]

File Language:

English

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