Development of a Method to Estimate Mouth-Level Benzo[a]pyrene Intake by Filter Analysis
Published Date:Oct 25 2011
Source:Cancer Epidemiol Biomarkers Prev. 21(1):39-44.
Pubmed Central ID:PMC4557766
Funding:YAD6/Intramural CDC HHS/United States
Benzo[a]pyrene (BaP) is one of the most potent carcinogens generated in cigarette smoke. During smoking, cigarette filters trap a significant portion of mainstream smoke benzo[a]pyrene. This trapped portion is proportional to what exits the end of the filter and is drawn into the mouth of smokers.
We developed a new method to estimate mouth-level BaP intake using filter analysis. In this analysis, cigarettes are smoked by a smoking machine using a variety of conditions to yield a range of mainstream smoke deliveries, which approximate a range of human puffing characteristics. Mainstream smoke BaP collected on Cambridge filter pads and the corresponding 1-cm mouth-end cigarette filter butts is extracted, purified by solid-phase extraction, and quantified by high-performance liquid chromatography coupled with a fluorescence detector. On the basis of the amount of BaP retained in cigarette butts and the amount collected on pads, we can relate them using a linear regression model.
Using this model and subsequently analyzing cigarette filters collected from smokers, we are able to estimate their mouth-level intakes, which smokers received when they consumed cigarettes. We made a series of measurements using research cigarettes and select commercial cigarettes having a wide range of machine smoke "tar" and nicotine deliveries.
In all cases, results indicate a linear relation of BaP between cigarette filter butts and Cambridge filter pads, with R2 ranging from 0.93 to 0.98.
This technique provides a noninvasive means to examine intake on a per cigarette basis to examine both exposure and behavioral aspects of smoking.
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