Development of a Method to Estimate Mouth-Level Benzo[a]pyrene Intake by Filter Analysis

Details

• Alternative Title:
  Cancer Epidemiol Biomarkers Prev
• Personal Author:
  Ding, Yan S. ; Chou, Theodore ; Abdul-Salaam, Shadeed ; Hearn, Bryan ; Watson, Clifford H.
• Description:
  Background
  
  Benzo[a]pyrene (BaP) is one of the most potent carcinogens generated in cigarette smoke. During smoking, cigarette filters trap a significant portion of mainstream smoke benzo[a]pyrene. This trapped portion is proportional to what exits the end of the filter and is drawn into the mouth of smokers.
  
  Methods
  
  We developed a new method to estimate mouth-level BaP intake using filter analysis. In this analysis, cigarettes are smoked by a smoking machine using a variety of conditions to yield a range of mainstream smoke deliveries, which approximate a range of human puffing characteristics. Mainstream smoke BaP collected on Cambridge filter pads and the corresponding 1-cm mouth-end cigarette filter butts is extracted, purified by solid-phase extraction, and quantified by high-performance liquid chromatography coupled with a fluorescence detector. On the basis of the amount of BaP retained in cigarette butts and the amount collected on pads, we can relate them using a linear regression model.
  
  Results
  
  Using this model and subsequently analyzing cigarette filters collected from smokers, we are able to estimate their mouth-level intakes, which smokers received when they consumed cigarettes. We made a series of measurements using research cigarettes and select commercial cigarettes having a wide range of machine smoke "tar" and nicotine deliveries.
  
  Conclusions
  
  In all cases, results indicate a linear relation of BaP between cigarette filter butts and Cambridge filter pads, with R2 ranging from 0.93 to 0.98.
  
  Impact
  
  This technique provides a noninvasive means to examine intake on a per cigarette basis to examine both exposure and behavioral aspects of smoking.
  
  
• Subjects:
  Benzo(a)pyrene Carcinogens Chromatography, High Pressure Liquid Filtration Humans Linear Models Reproducibility Of Results Smoke Spectrometry, Fluorescence Tobacco
• Source:
  Cancer Epidemiol Biomarkers Prev. 21(1):39-44.
• Pubmed ID:
  22028404
• Pubmed Central ID:
  PMC4557766
• Document Type:
  Journal Article
• Funding:
  YAD6/Intramural CDC HHS/United States
• Volume:
  21
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:21ffc153227ccca16a2f4c8c87a1659b53e3b08d6e82940155440a369f6b90f2
• Download URL:
  https://stacks.cdc.gov/view/cdc/34047/cdc_34047_DS1.pdf
• File Type:
  [PDF - 252.82 KB ]