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Development of a Method to Estimate Mouth-Level Benzo[a]pyrene Intake by Filter Analysis
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Details:
  • Pubmed ID:
    22028404
  • Pubmed Central ID:
    PMC4557766
  • Description:
    Background

    Benzo[a]pyrene (BaP) is one of the most potent carcinogens generated in cigarette smoke. During smoking, cigarette filters trap a significant portion of mainstream smoke benzo[a]pyrene. This trapped portion is proportional to what exits the end of the filter and is drawn into the mouth of smokers.

    Methods

    We developed a new method to estimate mouth-level BaP intake using filter analysis. In this analysis, cigarettes are smoked by a smoking machine using a variety of conditions to yield a range of mainstream smoke deliveries, which approximate a range of human puffing characteristics. Mainstream smoke BaP collected on Cambridge filter pads and the corresponding 1-cm mouth-end cigarette filter butts is extracted, purified by solid-phase extraction, and quantified by high-performance liquid chromatography coupled with a fluorescence detector. On the basis of the amount of BaP retained in cigarette butts and the amount collected on pads, we can relate them using a linear regression model.

    Results

    Using this model and subsequently analyzing cigarette filters collected from smokers, we are able to estimate their mouth-level intakes, which smokers received when they consumed cigarettes. We made a series of measurements using research cigarettes and select commercial cigarettes having a wide range of machine smoke "tar" and nicotine deliveries.

    Conclusions

    In all cases, results indicate a linear relation of BaP between cigarette filter butts and Cambridge filter pads, with R2 ranging from 0.93 to 0.98.

    Impact

    This technique provides a noninvasive means to examine intake on a per cigarette basis to examine both exposure and behavioral aspects of smoking.

  • Document Type:
  • Collection(s):
  • Funding:
    YAD6/Intramural CDC HHS/United States
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