Validation of an HPLC-MS/MS and Wipe Procedure for Mitomycin C Contamination

Details

• Alternative Title:
  J Chromatogr Sci
• Personal Author:
  B’Hymer, C. ; Connor, T.H. ; Stinson, D. ; Pretty, J.R.
• Description:
  A high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed for the determination of mitomycin C, an anticancer drug, from contamination on various surfaces. Mitomycin C is often used in various forms of intraperitoneal chemotherapy, and operating room healthcare worker exposure to this drug is possible. The surface testing method consisted of a wiping procedure utilizing a solution of 20/45/35 (v/v/v) of acetonitrile-isopropanol-water made 0.01 M in ammonium citrate (apparent pH 7.0). The wipe solutions were analyzed by means of HPLC-MS/MS using a reversed-phase gradient system and electrospray ionization in positive ion mode with a triple-quadrupole MS detector. Accuracy and precision of this method were demonstrated by a series of recovery studies of both spiked solutions and extracted wipes from various surfaces (stainless steel, vinyl and Formica(®)) spiked with known levels of mitomycin C. Recoveries of spiked solutions containing the analyte demonstrate mean recoveries (accuracy) ranged from 93 to 105%. Precision as measured by the relative standard deviation (% RSD) of multiple samples (n= 10) at each concentration level demonstrated values of 7.5% or less. The recoveries from spiked surfaces varied from 30 to 99%. The limit of detection for this methodology is ∼2 ng/100 cm(2) equivalent surface area, and the limit of quantitation is ∼6 ng/100 cm(2).
• Subjects:
  Article Chromatography, High Pressure Liquid Decontamination Environmental Monitoring HPLC-MS/MS Humans Limit Of Detection Linear Models Mitomycin Mitomycin C Occupational Exposure Reproducibility Of Results Surface Contamination Tandem Mass Spectrometry

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• Source:
  J Chromatogr Sci. 53(4):619-624.
• Pubmed ID:
  25129062
• Pubmed Central ID:
  PMC4556226
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  53
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:6dea71fc2d6b6b43236d37b3a76f56b38fd498c35b87f23d744f51e148866997
• Download URL:
  https://stacks.cdc.gov/view/cdc/34011/cdc_34011_DS1.pdf
• File Type:
  [PDF - 324.55 KB ]