Details:

Alternative Title:
J Chromatogr Sci
Personal Author:
B’Hymer, C. ; Connor, T.H. ; Stinson, D. ; Pretty, J.R.
B’Hymer, C. ; Connor, T.H. ; Stinson, D. ; Pretty, J.R. Less -
Description:
A high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed for the determination of mitomycin C, an anticancer drug, from contamination on various surfaces. Mitomycin C is often used in various forms of intraperitoneal chemotherapy, and operating room healthcare worker exposure to this drug is possible. The surface testing method consisted of a wiping procedure utilizing a solution of 20/45/35 (v/v/v) of acetonitrile-isopropanol-water made 0.01 M in ammonium citrate (apparent pH 7.0). The wipe solutions were analyzed by means of HPLC-MS/MS using a reversed-phase gradient system and electrospray ionization in positive ion mode with a triple-quadrupole MS detector. Accuracy and precision of this method were demonstrated by a series of recovery studies of both spiked solutions and extracted wipes from various surfaces (stainless steel, vinyl and Formica(®)) spiked with known levels of mitomycin C. Recoveries of spiked solutions containing the analyte demonstrate mean recoveries (accuracy) ranged from 93 to 105%. Precision as measured by the relative standard deviation (% RSD) of multiple samples (n= 10) at each concentration level demonstrated values of 7.5% or less. The recoveries from spiked surfaces varied from 30 to 99%. The limit of detection for this methodology is ∼2 ng/100 cm(2) equivalent surface area, and the limit of quantitation is ∼6 ng/100 cm(2).
Subject:
[+]
Article Chromatography, High Pressure Liquid Decontamination Environmental Monitoring HPLC-MS/MS Humans Limit Of Detection Linear Models Mitomycin Mitomycin C Occupational Exposure Reproducibility Of Results Surface Contamination Tandem Mass Spectrometry
Source:
J Chromatogr Sci. 53(4):619-624.
Pubmed ID:
25129062
Pubmed Central ID:
PMC4556226
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:6dea71fc2d6b6b43236d37b3a76f56b38fd498c35b87f23d744f51e148866997
Download URL:
https://stacks.cdc.gov/view/cdc/34011/cdc_34011_DS1.pdf
File Type:

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