Effect of lubricants and a vaginal spermicide gel on the detection of prostate specific antigen, a biomarker of semen exposure, using a quantitative (Abbott ARCHITECT) assay☆, ☆☆, ★

Details

• Alternative Title:
  Contraception
• Personal Author:
  Snead, Margaret C. ; Melendez, Johan H. ; Kourtis, Athena P. ; Chaney, Dorothy M. ; Brown, Teresa M. ; Black, Carolyn M. ; Mauck, Christine K. ; Schwartz, Jill L. ; Zenilman, Jonathan M. ; Jamieson, Denise J. ; Macaluso, Maurizio ; Doncel, Gustavo F.
• Description:
  Objectives
  
  Little is known about the effects of commonly used lubricants on detection of biomarkers of semen exposure. We investigated the in vitro effect of Gynol®, K-Y Jelly®, Replens®, Astroglide®, Carbopol, and Silicorel on quantitative detection of prostate specific antigen (PSA).
  
  Study Design
  
  A predetermined concentration of each of the gels was added to serially diluted semen samples. Additionally, serial dilutions of each of the gels were added to three different semen dilutions (high, medium, or low). The resulting samples were tested for PSA on the Abbott ARCHITECT System.
  
  Results
  
  When using the Abbott ARCHITECT system, the only products that inhibited PSA detection were Gynol® and Replens®. The inhibition caused by Gynol® was dose-dependent, but that of Replens was dose-independent. K-Y Jelly®-spiked samples had higher PSA values than controls.
  
  Conclusions
  
  Caution is warranted when using the Abbott quantitative assay for PSA detection as a biomarker of semen exposure in settings where Gynol®, Replens® or K-Y Jelly® might also have been used. Neither Astroglide® nor Silicorel inhibited PSA detection. Additional studies evaluating other vaginal products, including microbicides, and their effects on other assays, are needed. In vivo studies will be especially important to optimize PSA detection from clinical samples.
  
  Implications
  
  Researchers should consider the potential for specific lubricants or any vaginal products to affect the particular assay used for semen biomarker detection. The Abbott ARCHITECT’s total PSA assay should not be used with the product Replens. Caution is warranted when using the assay in settings where Gynol or K-Y jelly may have been used.
  
  
• Subjects:
  Article Female Humans Immunoassay Lubricants Luminescent Measurements Male Prostate-specific Antigen Semen Biomarkers Spermatocidal Agents Spermicide Vaginal Products

  More +
• Source:
  Contraception. 89(2):134-138.
• Pubmed ID:
  24314911
• Pubmed Central ID:
  PMC4554523
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  89
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:32b517c5d53e653a6c8afbdace60057a7489914c3f68526071d3dbeb9a72e804
• Download URL:
  https://stacks.cdc.gov/view/cdc/34000/cdc_34000_DS1.pdf
• File Type:
  [PDF - 747.12 KB ]