Details:

Alternative Title:
BMC Infect Dis
Publisher's site:
http://dx.doi.org/10.1186/1471-2334-3-12
Personal Author:
Vernon, Suzanne D; Farkas, Daniel H; Unger, Elizabeth R; Chan, Vivian; Miller, Donna L; ... More +
Vernon, Suzanne D; Farkas, Daniel H; Unger, Elizabeth R; Chan, Vivian; Miller, Donna L; Chen, Yin-Peng; Blackburn, Gary F; Reeves, William C; Less -
Description:
Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens. Methods Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes. Results Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples. Conclusions This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.
Subject:
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Biosensing Techniques
DNA Primers
DNA Probes, HPV
Humans
Microbiological Techniques
Oligonucleotide Array Sequence Analysis
Papillomaviridae

Source:
BMC Infect Dis. 2003; 3:12.;
Document Type:
Journal Article;
Funding:
Y1-CN-0101-01/CN/NCI NIH HHS/United States;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c719c2ce6115df41fe93e5cddec5677e63b6382b39a22bf8cce1e854f4465f2b
File Type:

Supporting Files

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