Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens
Published Date:Jun 19 2003
Source:BMC Infect Dis. 2003; 3:12.
Funding:Y1-CN-0101-01/CN/NCI NIH HHS/United States
We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.
Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.
This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.
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