Details:

Alternative Title:
BMC Infect Dis
Publisher's site:
http://dx.doi.org/10.1186/1471-2334-3-12
Personal Author:
Vernon, Suzanne D ; Farkas, Daniel H ; Unger, Elizabeth R ; Chan, Vivian ; Miller, Donna L ; ... More +
Vernon, Suzanne D ; Farkas, Daniel H ; Unger, Elizabeth R ; Chan, Vivian ; Miller, Donna L ; Chen, Yin-Peng ; Blackburn, Gary F ; Reeves, William C Less -
Description:
Background

We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.

Methods

Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.

Results

Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.

Conclusions

This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.


Subjects:
[+]
Biosensing Techniques DNA Primers DNA Probes, HPV Humans Microbiological Techniques Oligonucleotide Array Sequence Analysis Papillomaviridae
Source:
BMC Infect Dis. 2003; 3:12.
Document Type:
Journal Article
Funding:
Y1-CN-0101-01/CN/NCI NIH HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c719c2ce6115df41fe93e5cddec5677e63b6382b39a22bf8cce1e854f4465f2b
Download URL:
https://stacks.cdc.gov/view/cdc/3395/cdc_3395_DS1.pdf
File Type:

Supporting Files

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