Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens

Vernon, Suzanne D; Farkas, Daniel H; Unger, Elizabeth R; Chan, Vivian; Miller, Donna L; Chen, Yin-Peng; Blackburn, Gary F; Reeves, William C

doi:10.1186/1471-2334-3-12

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Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens

Published Date:

Jun 19 2003

Publisher's site:

http://dx.doi.org/10.1186/1471-2334-3-12 New Site Icon

Source:

BMC Infect Dis. 2003; 3:12.

[PDF-278.25 KB]

Details:

Alternative Title:

BMC Infect Dis

Personal Author:

Vernon, Suzanne D ; Farkas, Daniel H ; Unger, Elizabeth R ; Chan, Vivian ; Miller, Donna L ; ... More ▼

Description:

Background

We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.

Methods

Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.

Results

Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.

Conclusions

This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.

Subject:

[+]

Document Type:

Journal Article

Funding:

Y1-CN-0101-01/CN/NCI NIH HHS/United States

Collection(s):

CDC Public Access

Main Document Checksum:

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Supporting Files:

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