Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens

Details

• Alternative Title:
  BMC Infect Dis
• Personal Author:
  Vernon, Suzanne D ; Farkas, Daniel H ; Unger, Elizabeth R ; Chan, Vivian ; Miller, Donna L ; Chen, Yin-Peng ; Blackburn, Gary F ; Reeves, William C
• Description:
  Background
  
  We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
  
  Methods
  
  Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.
  
  Results
  
  Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.
  
  Conclusions
  
  This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.
  
  
• Subjects:
  Biosensing Techniques DNA Primers DNA Probes, HPV Humans Microbiological Techniques Oligonucleotide Array Sequence Analysis Papillomaviridae
• Source:
  BMC Infect Dis. 2003; 3:12.
• Document Type:
  Journal Article
• Funding:
  Y1-CN-0101-01/CN/NCI NIH HHS/United States
• Volume:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c719c2ce6115df41fe93e5cddec5677e63b6382b39a22bf8cce1e854f4465f2b
• Download URL:
  https://stacks.cdc.gov/view/cdc/3395/cdc_3395_DS1.pdf
• File Type:
  [PDF - 278.25 KB ]