Background

We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.

Methods

Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.

Results

Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.

Conclusions

This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.

CDC Public Access

Y1-CN-0101-01/CN/NCI NIH HHS/United States