Details:

Alternative Title:
BMC Microbiol
Personal Author:
Tiller, Rebekah V ; Gee, Jay E ; Lonsway, David R ; Gribble, Sonali ; Bell, Scott C ; ... More +
Tiller, Rebekah V ; Gee, Jay E ; Lonsway, David R ; Gribble, Sonali ; Bell, Scott C ; Jennison, Amy V ; Bates, John ; Coulter, Chris ; Hoffmaster, Alex R ; De, Barun K Less -
Description:
Background

Brucellosis is primarily a zoonotic disease caused by Brucella species. There are currently ten Brucella spp. including the recently identified novel B. inopinata sp. isolated from a wound associated with a breast implant infection. In this study we report on the identification of an unusual Brucella-like strain (BO2) isolated from a lung biopsy in a 52-year-old patient in Australia with a clinical history of chronic destructive pneumonia.

Results

Standard biochemical profiles confirmed that the unusual strain was a member of the Brucella genus and the full-length 16S rRNA gene sequence was 100% identical to the recently identified B. inopinata sp. nov. (type strain BO1T). Additional sequence analysis of the recA, omp2a and 2b genes; and multiple locus sequence analysis (MLSA) demonstrated that strain BO2 exhibited significant similarity to the B. inopinata sp. compared to any of the other Brucella or Ochrobactrum species. Genotyping based on multiple-locus variable-number tandem repeat analysis (MLVA) established that the BO2 and BO1Tstrains form a distinct phylogenetic cluster separate from the other Brucella spp.

Conclusion

Based on these molecular and microbiological characterizations, we propose that the BO2 strain is a novel lineage of the newly described B. inopinata species.


Subjects:
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Bacterial Typing Techniques Biopsy Brucella Brucellosis DNA, Bacterial Humans Lung Microbial Sensitivity Tests Middle Aged Phylogeny Pneumonia, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA
Source:
BMC Microbiol. 2010; 10:23.
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:befb382fdf6a9cc7e1ba7be9c27a8d9cc9a87ea532fa0fadb8d6cc0102a3fec0
Download URL:
https://stacks.cdc.gov/view/cdc/3366/cdc_3366_DS1.pdf
File Type:

Supporting Files

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