HCMV gB shares structural and functional properties with gB proteins from other herpesviruses

Sharma, Sapna; Wisner, Todd W.; Johnson, David C.; Heldwein, Ekaterina E.

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HCMV gB shares structural and functional properties with gB proteins from other herpesviruses

Supporting Files

October 22 2012
By Sharma, Sapna ; Wisner, Todd W. ; Johnson, David C. ; ...

File Language:

English

Details

Alternative Title:

Virology
Personal Author:

Sharma, Sapna ; Wisner, Todd W. ; Johnson, David C. ; Heldwein, Ekaterina E.
Description:

Glycoprotein B (gB) facilitates HCMV entry into cells by binding receptors and mediating membrane fusion. The crystal structures of gB ectodomains from HSV-1 and EBV are available, but little is known about the HCMV gB structure. Using multiangle light scattering and electron microscopy, we show here that HCMV gB ectodomain is a trimer with the overall shape similar to HSV-1 and EBV gB ectodomains. HCMV gB ectodomain forms rosettes similar to rosettes formed by EBV gB and the postfusion forms of other viral fusogens. Substitution of several bulky hydrophobic residues within the putative fusion loops with more hydrophilic residues reduced rosette formation and abolished cell fusion. We propose that like gB proteins from HSV-1 and EBV, HCMV gB has two internal hydrophobic fusion loops that likely interact with target membranes. Our work establishes structural and functional similarities between gB proteins from three subfamilies of herpesviruses.
Subjects:

Amino Acid Sequence Article Cytomegalovirus Entry Fusion Loop Glycoprotein B Glycosylation Herpesviridae Herpesvirus Herpesvirus 1, Human Herpesvirus 4, Human Mass Spectrometry Membrane Fusion Microscopy, Electron Models, Molecular Molecular Sequence Data Protein Conformation Rosette Viral Envelope Proteins
Source:

Virology. 2012; 435(2):239-249
Pubmed ID:

23089254
Pubmed Central ID:

PMC3534942
Document Type:

Journal Article
Funding:

R01 AI081517/AI/NIAID NIH HHS/United States ; 1DP20D001996/DP/NCCDPHP CDC HHS/United States ; R01AI081517/AI/NIAID NIH HHS/United States ; P30 AI042853/AI/NIAID NIH HHS/United States ; DP2 OD001996/OD/NIH HHS/United States
Volume:

435
Issue:

2
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:195663a88bbab72a99b4638dc944088247103b5d7e7eca50e2f08779ab92f22f
Download URL:

https://stacks.cdc.gov/view/cdc/33498/cdc_33498_DS1.pdf
File Type:

[PDF - 3.27 MB ]

File Language:

English

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