Rapid Identification of Pathogens from Positive Blood Cultures by Multiplex PCR using the FilmArray System

Details

• Alternative Title:
  Diagn Microbiol Infect Dis
• Personal Author:
  Blaschke, Anne J. ; Heyrend, Caroline ; Byington, Carrie L. ; Fisher, Mark A. ; Barker, Elizabeth ; Garrone, Nicholas F. ; Thatcher, Stephanie A. ; Pavia, Andrew T. ; Barney, Trenda ; Alger, Garrison D. ; Daly, Judy A. ; Ririe, Kirk M. ; Ota, Irene ; Poritz, Mark A.
• Description:
  Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.
• Subjects:
  Adult Article Bacteremia Bacteria Bacteriological Techniques Blood Blood Culture Bloodstream Infection Child Child, Preschool Drug Resistance, Bacterial FilmArray Humans Molecular Diagnostics Multiplex Polymerase Chain Reaction Polymerase Chain Reaction Sepsis

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• Source:
  Diagn Microbiol Infect Dis. 2012; 74(4):349-355
• Pubmed ID:
  22999332
• Pubmed Central ID:
  PMC3496814
• Document Type:
  Journal Article
• Funding:
  K24HD047249/HD/NICHD NIH HHSUnited States/ ; UL1 RR025764/RR/NCRR NIH HHSUnited States/ ; UL1RR025764/RR/NCRR NIH HHSUnited States/ ; 1U01AI082482/AI/NIAID NIH HHSUnited States/ ; U01 AI082184/AI/NIAID NIH HHSUnited States/ ; 1U18IP000303/IP/NCIRD CDC HHSUnited States/ ; 1K23AI079401/AI/NIAID NIH HHSUnited States/ ; U01A1061611/PHS HHSUnited States/ ; 1U01AI082184/AI/NIAID NIH HHSUnited States/ ; K23 AI079401/AI/NIAID NIH HHSUnited States/ ; U01 AI074419/AI/NIAID NIH HHSUnited States/ ; 1U01AI074419/AI/NIAID NIH HHSUnited States/ ; U18 IP000303/IP/NCIRD CDC HHSUnited States/ ; U01 AI082482/AI/NIAID NIH HHSUnited States/ ; K24 HD047249/HD/NICHD NIH HHSUnited States/
• Volume:
  74
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ac65b89f22cb5faf92f9db06aea9c15433e7ae4a8c49162e3675f51ef252949c
• Download URL:
  https://stacks.cdc.gov/view/cdc/33473/cdc_33473_DS1.pdf
• File Type:
  [PDF - 313.19 KB ]