Platypus TCRμ provides insight into the origins and evolution of a uniquely mammalian TCR locus1

Details

• Alternative Title:
  J Immunol
• Personal Author:
  Wang, Xinxin ; Parra, Zuly E. ; Miller, Robert D.
• Description:
  TCRμ is an unconventional TCR that was first discovered in marsupials and appears to be absent from placental mammals and nonmammals. In this study, we show that TCRμ is also present in the duckbill platypus, an egg-laying monotreme, consistent with TCRμ being ancient and present in the last common ancestor of all extant mammals. As in marsupials, platypus TCRμ is expressed in a form containing double V domains. These V domains more closely resemble Ab V than that of conventional TCR. Platypus TCRμ differs from its marsupial homolog by requiring two rounds of somatic DNA recombination to assemble both V exons and has a genomic organization resembling the likely ancestral form of the receptor genes. These results demonstrate that the ancestors of placental mammals would have had TCRμ but it has been lost from this lineage.
• Subjects:
  Amino Acid Sequence Animals Base Sequence Evolution, Molecular Genetic Loci Molecular Sequence Data Phylogeny Platypus Receptors, Antigen, T-Cell Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Somatic Hypermutation, Immunoglobulin

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• Source:
  J Immunol. 187(10):5246-5254
• Pubmed ID:
  21976776
• Pubmed Central ID:
  PMC3208081
• Document Type:
  Journal Article
• Funding:
  P20 RR018754/RR/NCRR NIH HHSUnited States/ ; P20 RR018754-09/RR/NCRR NIH HHSUnited States/ ; IP20RR18754/IP/NCIRD CDC HHSUnited States/
• Volume:
  187
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:4989b02ee2c9463d5b15b650b2a8ac9c374d0d13700ceeb46c140dfaacd9fea8
• Download URL:
  https://stacks.cdc.gov/view/cdc/33268/cdc_33268_DS1.pdf
• File Type:
  [PDF - 2.01 MB ]