Are Mitochondrial Reactive Oxygen Species Required for Autophagy?

Details

• Alternative Title:
  Biochem Biophys Res Commun
• Personal Author:
  Jiang, Jianfei ; Maeda, Akihiro ; Ji, Jing ; Baty, Catherine J. ; Watkins, Simon C. ; Greenberger, Joel S. ; Kagan, Valerian E.
• Description:
  Reactive oxygen species (ROS) are said to participate in the autophagy signaling. Supporting evidence is obscured by interference of autophagy and apoptosis, whereby the latter heavily relies on ROS signaling. To dissect autophagy from apoptosis we knocked down expression of cytochrome c, the key component of mitochondria-dependent apoptosis, in HeLa cells using shRNA. In cytochrome c deficient HeLa1.2 cells, electron transport was compromised due to the lack of electron shuttle between mitochondrial respiratory complexes III and IV. A rapid and robust LC3-I/II conversion and mitochondria degradation were observed in HeLa1.2 cells treated with staurosporine (STS). Neither generation of superoxide nor accumulation of H(2)O(2) was detected in STS-treated HeLa1.2 cells. A membrane permeable antioxidant, PEG-SOD, plus catalase exerted no effect on STS-induced LC3-I/II conversion and mitochondria degradation. Further, STS caused autophagy in mitochondria DNA-deficient ρ° HeLa1.2 cells in which both electron transport and ROS generation were completely disrupted. Counter to the widespread view, we conclude that mitochondrial ROS are not required for the induction of autophagy.
• Subjects:
  Autophagy Cytochromes C DNA, Mitochondrial HeLa Cells Humans Mitochondria Reactive Oxygen Species Staurosporine
• Source:
  Biochem Biophys Res Commun. 2011; 412(1):55-60.
• Pubmed ID:
  21806968
• Pubmed Central ID:
  PMC3171134
• Document Type:
  Journal Article
• Funding:
  HL094488/HL/NHLBI NIH HHS/United States ; HL70755/HL/NHLBI NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; R01 HL070755/HL/NHLBI NIH HHS/United States ; R01 HL070755-07/HL/NHLBI NIH HHS/United States ; R01 HL070755-08/HL/NHLBI NIH HHS/United States ; R01 HL094488/HL/NHLBI NIH HHS/United States ; R01 HL094488-01A1/HL/NHLBI NIH HHS/United States ; R01 HL094488-02/HL/NHLBI NIH HHS/United States ; U19 A8068021/PHS HHS/United States ; U19 AI068021/AI/NIAID NIH HHS/United States ; U19 AI068021-06/AI/NIAID NIH HHS/United States ; U19 AI068021-07/AI/NIAID NIH HHS/United States ; U19-AI068021/AI/NIAID NIH HHS/United States
• Volume:
  412
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ce6bc52dbcca96374c595876149be48c7c4bcc2a9ce997cb131c29daf9bca5a8
• Download URL:
  https://stacks.cdc.gov/view/cdc/33245/cdc_33245_DS1.pdf
• File Type:
  [PDF - 2.43 MB ]