International Mycoplasma pneumoniae typing study: interpretation of M. pneumoniae multilocus variable-number tandem-repeat analysis

Details

• Alternative Title:
  New Microbes New Infect
• Personal Author:
  Chalker, V.J. ; Pereyre, S. ; Dumke, R. ; Winchell, J. ; Khosla, P. ; Sun, H. ; Yan, C. ; Vink, C. ; Bébéar, C.
• Description:
  Typing of Mycoplasma pneumoniae by multiple-locus variable-number tandem repeat analysis (MLVA) is increasingly in use. However, no specific internationally agreed guidance is available. Thirty M. pneumoniae DNA samples including serial dilutions of a type strain were sent to six international laboratories to perform MLVA and results were compared. Good correlation was observed, indicating that this methodology can be robustly performed in multiple sites. However, differences due to interpretation of fragment size, repeat sequence identification and repeat numbering led to inconsistency in the final profiles assigned by laboratories. We propose guidelines for interpreting M. pneumoniae MLVA typing and assigning the number of repeats.
• Subjects:
  Mycoplasma pneumoniae/genetics
• Source:
  New Microbes New Infect. 2015; 7:37-40.
• Pubmed ID:
  26236493
• Pubmed Central ID:
  PMC4501435
• Document Type:
  Journal Article
• Volume:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:71426005c7b720ac948e5cacdbd0b371986daf5faf7547ff94cc6cb1c8b8e021
• Download URL:
  https://stacks.cdc.gov/view/cdc/32693/cdc_32693_DS1.pdf
• File Type:
  [PDF - 94.43 KB ]