Nitroreductase-Activatable Morpholino Oligonucleotides
for in Vivo Gene Silencing

Yamazoe, Sayumi; McQuade, Lindsey
E.; Chen, James K.

doi:10.1021/cb500429u

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Nitroreductase-Activatable Morpholino Oligonucleotides for in Vivo Gene Silencing

Published Date:

Jul 28 2014

Publisher's site:

http://dx.doi.org/10.1021/cb500429u New Site Icon

Source:

ACS Chem Biol. 2014; 9(9):1985-1990.

[PDF-1.40 MB]

Details:

Personal Authors:

Yamazoe, Sayumi ; McQuade, Lindsey E. ; Chen, James K. ;

Keywords:

[+]

Pubmed ID:

25069083

Pubmed Central ID:

PMC4168795

Description:

Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. We describe here a new class of caged morpholino oligonucleotides that can be activated by the bacterial nitroreductase NfsB. We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

DP1 HD075622/DP/NCCDPHP CDC HHS/United States
P50 GM107615/GM/NIGMS NIH HHS/United States
P50 GM107615/GM/NIGMS NIH HHS/United States
R01 GM087292/GM/NIGMS NIH HHS/United States
R21 HD078385/HD/NICHD NIH HHS/United States

Supporting Files:

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