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Nitroreductase-Activatable Morpholino Oligonucleotides for in Vivo Gene Silencing
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Details:
  • Pubmed ID:
    25069083
  • Pubmed Central ID:
    PMC4168795
  • Description:
    Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. We describe here a new class of caged morpholino oligonucleotides that can be activated by the bacterial nitroreductase NfsB. We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting.

  • Document Type:
  • Collection(s):
  • Funding:
    DP1 HD075622/DP/NCCDPHP CDC HHS/United States
    P50 GM107615/GM/NIGMS NIH HHS/United States
    P50 GM107615/GM/NIGMS NIH HHS/United States
    R01 GM087292/GM/NIGMS NIH HHS/United States
    R21 HD078385/HD/NICHD NIH HHS/United States
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