Nitroreductase-Activatable Morpholino Oligonucleotides
for in Vivo Gene Silencing

Yamazoe, Sayumi; McQuade, Lindsey
E.; Chen, James K.

doi:10.1021/cb500429u

i

Nitroreductase-Activatable Morpholino Oligonucleotides for in Vivo Gene Silencing

Supporting Files

Jul 28 2014
By Yamazoe, Sayumi ; McQuade, Lindsey E. ; Chen, James K.

Details

Alternative Title:

ACS Chem Biol
Personal Author:

Yamazoe, Sayumi ; McQuade, Lindsey E. ; Chen, James K.
Description:

Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. We describe here a new class of caged morpholino oligonucleotides that can be activated by the bacterial nitroreductase NfsB. We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting.
Subjects:

Letters
Source:

ACS Chem Biol. 2014; 9(9):1985-1990.
Pubmed ID:

25069083
Pubmed Central ID:

PMC4168795
Document Type:

Journal Article
Funding:

DP1 HD075622/DP/NCCDPHP CDC HHS/United States ; P50 GM107615/GM/NIGMS NIH HHS/United States ; P50 GM107615/GM/NIGMS NIH HHS/United States ; R01 GM087292/GM/NIGMS NIH HHS/United States ; R21 HD078385/HD/NICHD NIH HHS/United States
Volume:

9
Issue:

9
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:fce756927db90c9ae645308a018e72bd8c8cac6bfca81233a93c5cba21e28b94
Download URL:

https://stacks.cdc.gov/view/cdc/32573/cdc_32573_DS1.pdf
File Type:

[PDF - 1.40 MB ]

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