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Interfacing Microbial Styrene Production with a Biocompatible Cyclopropanation Reaction**
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Details:
  • Pubmed ID:
    25925138
  • Pubmed Central ID:
    PMC4494747
  • Description:
    The introduction of new reactivity into living organisms is a major challenge in synthetic biology. Despite an increasing interest in both the development of small-molecule catalysts that are compatible with aqueous media and the engineering of enzymes to perform new chemistry in vitro, the integration of non-native reactivity into metabolic pathways for small-molecule production has been underexplored. Herein we report a biocompatible iron(III) phthalocyanine catalyst capable of efficient olefin cyclopropanation in the presence of a living microorganism. By interfacing this catalyst with E. coli engineered to produce styrene, we synthesized non-natural phenyl cyclopropanes directly from D-glucose in single-vessel fermentations. This process is the first example of the combination of nonbiological carbene-transfer reactivity with cellular metabolism for small-molecule production.

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  • Funding:
    DP2 GM105434/DP/NCCDPHP CDC HHS/United States
    DP2 GM105434/GM/NIGMS NIH HHS/United States
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