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Acid-fast direct smear microscopy
  • Published Date:
    July 2000
Filetype[PDF-457.42 KB]

  • Corporate Authors:
    Instituto Nacional de Diagnóstico y Referencia Epidemiológicos (Mexico) ; World Health Organization ; International Union against Tuberculosis and Lung Disease ; ... More ▼
  • Description:
    "This program is designed to assist laboratory technicians in performning direct AFB microscopy. The program content is intended to complement laborartory manuals by demonstrating appropriate technique, emphasizing standard methods, and promoting consistent reading and reporting of smear results." --User's guide

    NPIN 31108: This information kit presents a laboratory training program for laboratory technicians who perform or supervise direct acid-fast bacilli (AFB) microscopy sputum testing for tuberculosis (TB). It uses the methods and practices recommended by the World Health Organization (WHO) and the International Union Against Tuberculosis and Lung Disease (IUATLD). The information kit explains the merits of AFB and discusses the mode of transmission of TB to emphasize the danger of working with the disease and the importance of biosafety in the laboratory. It explains specimen collection, laboratory arrangement, transportation and labeling of specimens, recording in a laboratory register, smear preparation, staining procedures, microscope examination, reporting and recording, and quality control in the TB laboratory. A quiz is included to measure what was learned from the video and other training materials.

    This laboratory training program was produced by the Association of Public Health Laboratories through a cooperative agreement with the Centers for Disease Control and Prevention #U60/CCU303019-12

    Forty-one booklet from a kit consisting of: 1 videocassette (31 min.): sd., col. ; 1/2 in.; 1 user's guide (41 p.) : ill. ; 22 cm.; 1 computer laser optical disc ; 4 3/4 in.

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