Endothelial Cell Cytotoxicity of Cotton Bracts Tannin and Aqueous Cotton Bracts Extract: Tannin Is the Predominant Cytotoxin Present in Aqueous Cotton Bracts Extract

Details

• Journal Article:
  Environmental Health Perspectives
• Personal Author:
  Johnson, Christopher M. ; Hanson, Michelle N. ; Rohrbach, Michael S.
• Description:
  Cultured porcine thoracic aortic and pulmonary arterial endothelial cells were used to determine whether the tannin present in cotton bract exerts a cytotoxic action on endothelial cells. Six hours of exposure to various concentrations of tannin isolated from aqueous cotton bract extracts induced lethal cell injury, measured in terms of release of chromium-51 from the cell cultures. Light microscopy and scanning electron microscopy demonstrated that exposure to cotton bract tannin also induced time dependent changes in the morphology of the endothelial cells. Changes indicative of the collapse of the cell membrane over the cytoskeleton appeared to signal the onset of cellular lethal injury. Comparison of the toxic effects of cotton bract tannin and aqueous cotton bract extracts on endothelial cells yielded similar cytotoxicity curves. The bract curve was less steep than the tannin curve, but both products induced a maximal release of chromium-51 in concentrations of 100 micrograms per milliliter, with an equal degree of maximal lethal cellular injury. The authors conclude that bract tannin exerts a severe toxic effect at the level of the pulmonary endothelial cells and that this effect is not influenced by other water soluble products present in the bracts. [Description provided by NIOSH]
• Subjects:
  Aorta, Thoracic Blood Byssinosis Cells, Cultured Cell Survival Cytology Cytotoxins Dust Endothelium Gossypium Laboratories Occupational Health Pulmonary Artery Respiratory Tract Diseases Safety Tannins Toxicology

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• Keywords:
  NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; In-vitro-studies; Laboratory-animals; Blood-vessels; Cotton-dust; Dose-response; Toxicology; Cytopathology; Cell-cultures; Cell-damage; Microscopy; Cytotoxic-effects
• Source:
  Environ Health Perspect 1986 Apr; 66:97-103
• ISSN:
  0091-6765
• Document Type:
  Text
• Funding:
  HL-07111/HL/NHLBI NIH HHS/United States ; HL-28669/HL/NHLBI NIH HHS/United States ; OH-02116/OH/NIOSH CDC HHS/United States
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  7 pdf pages
• Volume:
  66
• NIOSHTIC Number:
  nn:00167559
• Contact Point Address:
  Internal Medicine Mayo Foundation 200 First Street S W Rochester, MN 55905
• Federal Fiscal Year:
  1986
• Performing Organization:
  Mayo Foundation, Rochester, Minnesota
• Peer Reviewed:
  True
• Collection(s):
  National Institute for Occupational Safety and Health CDC Public Access
• Main Document Checksum:
  urn:sha-512:cbe4dccec3fa09a8407884495829da3a76a2afe0401684d5ca7b29e5dcd2b9366fca4ee101f90218a3b2beefb04948b1fe92114355b8e328eb8b2e62db595d93
• Download URL:
  https://stacks.cdc.gov/view/cdc/30773/cdc_30773_DS1.pdf
• File Type:
  [PDF - 3.68 MB ]