CNS Myelination Requires Cytoplasmic Dynein Function

Details

• Alternative Title:
  Dev Dyn
• Personal Author:
  Yang, Michele L. ; Shin, Jimann ; Kearns, Christina A. ; Langworthy, Melissa M. ; Snell, Heather ; Walker, Macie B. ; Appel, Bruce
• Description:
  Background
  
  Cytoplasmic dynein provides the main motor force for minus-end-directed transport of cargo on microtubules. Within the vertebrate central nervous system (CNS), proliferation, neuronal migration and retrograde axon transport are among the cellular functions known to require dynein. Accordingly, mutations of DYNC1H1, which encodes the heavy chain subunit of cytoplasmic dynein, have been linked to developmental brain malformations and axonal pathologies. Oligodendrocytes, the myelinating glial cell type of the CNS, migrate from their origins to their target axons and subsequently extend multiple long processes that ensheath axons with specialized insulating membrane. These processes are filled with microtubules, which facilitate molecular transport of myelin components. However, whether oligodendrocytes require cytoplasmic dynein to ensheath axons with myelin is not known.
  
  Results
  
  We identified a mutation of zebrafish dync1h1 in a forward genetic screen that caused a deficit of oligodendrocytes. Using in vivo imaging and gene expression analyses, we additionally found evidence that dync1h1 promotes axon ensheathment and myelin gene expression.
  
  Conclusions
  
  In addition to its well known roles in axon transport and neuronal migration, cytoplasmic dynein contributes to neural development by promoting myelination.
  
  
• Subjects:
  Animals Article Axon Axonal Transport Axons Brain Cytoplasmic Dyneins Dynein Gene Expression Regulation, Developmental Mutation Myelination Myelin Sheath Oligodendrocyte Oligodendroglia Zebrafish Zebrafish Proteins

  More +
• Source:
  Dev Dyn. 244(2):134-145.
• Pubmed ID:
  25488883
• Pubmed Central ID:
  PMC4368448
• Document Type:
  Journal Article
• Funding:
  P30 NS048154/NS/NINDS NIH HHS/United States ; P30 NS08154/NS/NINDS NIH HHS/United States ; R01 NS046668/NS/NINDS NIH HHS/United States ; R01 NS046668/NS/NINDS NIH HHS/United States ; T32 MN015442/MN/OMHHE CDC HHS/United States
• Volume:
  244
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:873e5417ee86806f306179d1fcf1db47b025678cea8cdab945680fb3747ba200
• Download URL:
  https://stacks.cdc.gov/view/cdc/30338/cdc_30338_DS1.pdf
• File Type:
  [PDF - 1.07 MB ]