Sequential Gene Silencing Using Wavelength-Selective Caged Morpholinos**

Details

• Alternative Title:
  Angew Chem Int Ed Engl
• Personal Author:
  Yamazoe, Sayumi ; Liu, Qingyang ; McQuade, Lindsey E. ; Deiters, Alexander ; Chen, James K.
• Description:
  Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse-genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.
• Subjects:
  Amino Acid Sequence Animals Antisense Oligonucleotides Article Caged Compounds Developmental Biology Gene Expression Gene Silencing Molecular Structure Morpholinos Zebrafish

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• Source:
  Angew Chem Int Ed Engl. 2013; 53(38):10114-10118.
• Pubmed ID:
  25130695
• Pubmed Central ID:
  PMC4206551
• Document Type:
  Journal Article
• Funding:
  DP1 HD075622/DP/NCCDPHP CDC HHS/United States ; P50 GM107615/GM/NIGMS NIH HHS/United States ; R01 GM079114/GM/NIGMS NIH HHS/United States ; R01 GM087292/GM/NIGMS NIH HHS/United States ; R21 HD078385/HD/NICHD NIH HHS/United States
• Volume:
  53
• Issue:
  38
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:daff239b6094cded8b740436ff35c7a45e3ee7fe959184103fcbc05b9228c22a
• Download URL:
  https://stacks.cdc.gov/view/cdc/30151/cdc_30151_DS1.pdf
• File Type:
  [PDF - 795.37 KB ]