Details:

Alternative Title:
Reproduction
Publisher's site:
http://dx.doi.org/10.1530/REP-13-0663
Personal Author:
Grazul-Bilska, Anna T. ; Johnson, Mary Lynn ; Borowicz, Pawel P. ; Bilski, Jerzy J. ; Cymbaluk, Taylor ; ... More +
Grazul-Bilska, Anna T. ; Johnson, Mary Lynn ; Borowicz, Pawel P. ; Bilski, Jerzy J. ; Cymbaluk, Taylor ; Norberg, Spencer ; Redmer, Dale A. ; Reynolds, Lawrence P. Less -
Description:
Utero-placental growth and vascular development are critical for pregnancy establishment that may be altered by various factors including assisted reproductive technologies (ART), nutrition, or others, leading to compromised pregnancy. We hypothesized that placental vascularization and expression of angiogenic factors are altered early in pregnancies after transfer of embryos created using selected ART methods. Pregnancies were achieved through natural mating (NAT), or transfer of embryos from NAT (NAT-ET), or IVF or in vitro activation (IVA). Placental tissues were collected on day 22 of pregnancy. In maternal caruncles (CAR), vascular cell proliferation was less (P<0.05) for IVA than other groups. Compared with NAT, density of blood vessels was less (P<0.05) for IVF and IVA in fetal membranes (FM) and for NAT-ET, IVF, and IVA in CAR. In FM, mRNA expression was decreased (P<0.01-0.08) in NAT-ET, IVF, and IVA compared with NAT for vascular endothelial growth factor (VEGF) and its receptor FLT1, placental growth factor (PGF), neuropilin 1 (NP1) and NP2, angiopoietin 1 (ANGPT1) and ANGPT2, endothelial nitric oxide synthase 3 (NOS3), hypoxia-inducible factor 1A (HIF1A), fibroblast growth factor 2 (FGF2), and its receptor FGFR2. In CAR, mRNA expression was decreased (P<0.01-0.05) in NAT-ET, IVF, and IVA compared with NAT for VEGF, FLT1, PGF, ANGPT1, and TEK. Decreased mRNA expression for 12 of 14 angiogenic factors across FM and CAR in NAT-ET, IVF, and IVA pregnancies was associated with reduced placental vascular development, which would lead to poor placental function and compromised fetal and placental growth and development.
Subject:
[+]
Animals
Article
Embryo, Mammalian
Embryo Transfer
Female
Fertilization In Vitro
Models, Animal
Neovascularization, Physiologic
Placenta
Placentation
Pregnancy
Pregnancy, Animal
Pregnancy Proteins
Reproductive Techniques, Assisted
Sheep
Time Factors

Source:
Reproduction. 147(5):639-648.
Pubmed ID:
24472816
Pubmed Central ID:
PMC3981880
Document Type:
Journal Article
Funding:
R01 HL064141/HL/NHLBI NIH HHS/United States ; HL64141/HL/NHLBI NIH HHS/United States ; ND01712/ND/ONDIEH CDC HHS/United States ; ND1727/ND/ONDIEH CDC HHS/United States
R01 HL064141/HL/NHLBI NIH HHS/United States ; HL64141/HL/NHLBI NIH HHS/United States ; ND01712/ND/ONDIEH CDC HHS/United States ; ND1727/ND/ONDIEH CDC HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:95ce54c10b4695de1ad10d2564784c81354a53cf20162da34cea688742ddd4fe
File Type:

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