Details:

Alternative Title:
Nanoscale
Personal Author:
Gao, Lizeng ; Giglio, Krista M. ; Nelson, Jacquelyn L. ; Sondermann, Holger ; Travis, Alexander J.
Gao, Lizeng ; Giglio, Krista M. ; Nelson, Jacquelyn L. ; Sondermann, Holger ; Travis, Alexander J. Less -
Description:
Hydrogen peroxide (H2O2) is a "green chemical" that has various cleaning and disinfectant uses, including as an anti-bacterial agent for hygienic and medical treatments. However, its efficacy is limited against biofilm-producing bacteria, because of poor penetration into the protective, organic matrix. Here we show new applications for ferromagnetic nanoparticles (Fe3O4, MNPs) with peroxidase-like activity in potentiating the efficacy of H2O2 in biofilm degradation and prevention. Our data show that MNPs enhanced oxidative cleavage of biofilm components (model nucleic acids, proteins, and oligosaccharides) in the presence of H2O2. When challenged with live, biofilm-producing bacteria, the MNP-H2O2 system efficiently broke down the existing biofilm and prevented new biofilms from forming, killing both planktonic bacteria and those within the biofilm. By enhancing oxidative cleavage of various substrates, the MNP-H2O2 system provides a novel strategy for biofilm elimination, and other applications utilizing oxidative breakdown.
Subjects:
[+]
Anti-Bacterial Agents Article Bacteria Biocompatible Materials Biofilms DNA DNA Cleavage Ferrosoferric Oxide Hydrogen Peroxide Magnetite Nanoparticles Oxidation-Reduction Peroxidases Polysaccharides Proteins Pseudomonas Aeruginosa
Source:
Nanoscale. 2012; 6(5):2588-2593.
Pubmed ID:
24468900
Pubmed Central ID:
PMC3951791
Document Type:
Journal Article
Funding:
8DP1-EB016541/DP/NCCDPHP CDC HHS/United States ; DP1 EB016541/EB/NIBIB NIH HHS/United States ; R01 AI097307/AI/NIAID NIH HHS/United States
8DP1-EB016541/DP/NCCDPHP CDC HHS/United States ; DP1 EB016541/EB/NIBIB NIH HHS/United States ; R01 AI097307/AI/NIAID NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:52412f37a3f2b86904b5ff1961e5f1e8d3cc8cdc51e30a6cd06211afa985df4d
Download URL:
https://stacks.cdc.gov/view/cdc/29879/cdc_29879_DS1.pdf
File Type:

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