Ferromagnetic nanoparticles with peroxidase-like activity enhance the cleavage of biological macromolecules for biofilm elimination

Details

• Alternative Title:
  Nanoscale
• Personal Author:
  Gao, Lizeng ; Giglio, Krista M. ; Nelson, Jacquelyn L. ; Sondermann, Holger ; Travis, Alexander J.
• Description:
  Hydrogen peroxide (H2O2) is a "green chemical" that has various cleaning and disinfectant uses, including as an anti-bacterial agent for hygienic and medical treatments. However, its efficacy is limited against biofilm-producing bacteria, because of poor penetration into the protective, organic matrix. Here we show new applications for ferromagnetic nanoparticles (Fe3O4, MNPs) with peroxidase-like activity in potentiating the efficacy of H2O2 in biofilm degradation and prevention. Our data show that MNPs enhanced oxidative cleavage of biofilm components (model nucleic acids, proteins, and oligosaccharides) in the presence of H2O2. When challenged with live, biofilm-producing bacteria, the MNP-H2O2 system efficiently broke down the existing biofilm and prevented new biofilms from forming, killing both planktonic bacteria and those within the biofilm. By enhancing oxidative cleavage of various substrates, the MNP-H2O2 system provides a novel strategy for biofilm elimination, and other applications utilizing oxidative breakdown.
• Subjects:
  Anti-Bacterial Agents Bacteria Biocompatible Materials Biofilms DNA DNA Cleavage Ferrosoferric Oxide Hydrogen Peroxide Magnetite Nanoparticles Oxidation-Reduction Peroxidases Polysaccharides Proteins Pseudomonas Aeruginosa

  More +
• Source:
  Nanoscale. 2012; 6(5):2588-2593
• Pubmed ID:
  24468900
• Pubmed Central ID:
  PMC3951791
• Document Type:
  Journal Article
• Funding:
  DP1 EB016541/EB/NIBIB NIH HHSUnited States/ ; R01 AI097307/AI/NIAID NIH HHSUnited States/ ; 8DP1-EB016541/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  6
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:52412f37a3f2b86904b5ff1961e5f1e8d3cc8cdc51e30a6cd06211afa985df4d
• Download URL:
  https://stacks.cdc.gov/view/cdc/29879/cdc_29879_DS1.pdf
• File Type:
  [PDF - 1.51 MB ]