Single Vehicular Delivery of siRNA and Small Molecules to Control Stem Cell Differentiation

Details

• Alternative Title:
  J Am Chem Soc
• Personal Author:
  Shah, Shreyas ; Solanki, Aniruddh ; Sasmal, Pijus K. ; Lee, Ki-Bum
• Description:
  Achieving a controlled and reproducible means to direct stem cell differentiation is the single most critical concern scientists have been trying to address since the discovery of stem cells. In this regard, the use of small molecules and RNA interference offers unique advantages by targeting different cellular mechanisms. Our cyclodextrin-modified dendritic polyamine construct (termed DexAM) combines the unique properties of two distinct chemical moieties in a single delivery vehicle. DexAM is a single vehicle that not only solubilizes hydrophobic small molecules in physiological solutions but also forms complexes with siRNA molecules, making it an attractive delivery system for controlling stem cell differentiation. Herein, we report the synthesis and application of DexAM to simultaneously deliver hydrophobic small molecules and siRNA into neural stem cells to significantly enhance their neuronal differentiation.
• Subjects:
  Article Cell Differentiation Cell Line Cyclodextrins Dose-Response Relationship, Drug Drug Delivery Systems Humans Hydrophobic And Hydrophilic Interactions Models, Molecular Molecular Structure Neural Stem Cells Polyamines RNA, Small Interfering Structure-Activity Relationship

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• Source:
  J Am Chem Soc. 135(42):15682-15685.
• Pubmed ID:
  24106916
• Pubmed Central ID:
  PMC3841293
• Document Type:
  Journal Article
• Funding:
  DP2 OD006462/OD/NIH HHS/United States ; R21 NS085569/NS/NINDS NIH HHS/United States ; 1DP20D006462-01/DP/NCCDPHP CDC HHS/United States
• Volume:
  135
• Issue:
  42
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c0ef42f1f51e912f045708696d7d21362c6b3b416ad19a5331d480eebbc032e4
• Download URL:
  https://stacks.cdc.gov/view/cdc/29788/cdc_29788_DS1.pdf
• File Type:
  [PDF - 1.11 MB ]