Functional inhibition of UQCRB suppresses angiogenesis in zebrafish

Details

• Alternative Title:
  Biochem Biophys Res Commun
• Personal Author:
  Cho, Yoon Sun ; Jung, Hye Jin ; Seok, Seung Hyeok ; Payumo, Alexander Y. ; Chen, James K. ; Kwon, Ho Jeong
• Description:
  As a subunit of mitochondrial complex III, UQCRB plays an important role in complex III stability, electron transport, and cellular oxygen sensing. Herein, we report UQCRB function regarding angiogenesis in vivo with the zebrafish (Danio rerio). UQCRB knockdown inhibited angiogenesis in zebrafish leading to the suppression of VEGF expression. Moreover, the UQCRB-targeting small molecule terpestacin also inhibited angiogenesis and VEGF levels in zebrafish, supporting the role of UQCRB in angiogenesis. Collectively, UQCRB loss of function by either genetic and pharmacological means inhibited angiogenesis, indicating that UQCRB plays a key role in this process and can be a prognostic marker of angiogenesis- and mitochondria-related diseases.
• Subjects:
  Amino Acid Sequence Angiogenesis Animals Article Bicyclo Compounds Carrier Proteins Dose-Response Relationship, Drug Embryo, Nonmammalian Embryonic Development Gene Expression Regulation, Developmental Gene Knockdown Techniques Mitochondria Molecular Sequence Data Morpholinos Neovascularization, Physiologic Prognosis UQCRB Vascular Endothelial Growth Factor A Zebrafish

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• Source:
  Biochem Biophys Res Commun. 2013; 433(4):396-400.
• Pubmed ID:
  23454382
• Pubmed Central ID:
  PMC3691074
• Document Type:
  Journal Article
• Funding:
  DP1 HD075622/HD/NICHD NIH HHS/United States ; DP1HD075622/DP/NCCDPHP CDC HHS/United States
• Volume:
  433
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:901ed1113e5758f9db5b8ff7cadcd49cbb1c0b336eb12d844e7f9b5b677e068e
• Download URL:
  https://stacks.cdc.gov/view/cdc/29680/cdc_29680_DS1.pdf
• File Type:
  [PDF - 392.16 KB ]