Genome Mining of a Prenylated and Immunosuppressive Polyketide from Pathogenic Fungi

Details

• Alternative Title:
  Org Lett
• Personal Author:
  Chooi, Yit-Heng ; Fang, Jinxu ; Liu, Hong ; Filler, Scott G. ; Wang, Pin ; Tang, Yi
• Description:
  Activation of the polycyclic polyketide prenyltransferase (pcPTase)-containing silent clusters in Aspergillus fumigatus and Neosartorya fischeri led to isolation of a new metabolite neosartoricin (3). The structure of 3 was solved by X-ray crystallography and NMR to be a prenylated anthracenone. 3 exhibits T-cell antiproliferative activity with an IC(50) of 3 μM, suggestive of a physiological role as an immunosuppressive agent.
• Subjects:
  Animals Article Aspergillus Fumigatus Crystallography, X-Ray Dimethylallyltranstransferase Fungi Genome HeLa Cells Humans Immunosuppressive Agents Macrophages Mice Molecular Conformation Neosartorya Nuclear Magnetic Resonance, Biomolecular Polyketides Prenylation

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• Source:
  Org Lett. 15(4):780-783
• Pubmed ID:
  23368997
• Pubmed Central ID:
  PMC3576815
• Document Type:
  Journal Article
• Funding:
  DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; UL1 TR000124/TR/NCATS NIH HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/ ; R01AI073829/AI/NIAID NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; R01 AI073829/AI/NIAID NIH HHSUnited States/
• Volume:
  15
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:39d2f6276f2a469a1320b99a496fb495f475d5df7729ea9d371fa70abdf776f0
• Download URL:
  https://stacks.cdc.gov/view/cdc/29623/cdc_29623_DS1.pdf
• File Type:
  [PDF - 1.03 MB ]