Details:

Alternative Title:
BMC Infect Dis
Publisher's site:
http://dx.doi.org/10.1186/1471-2334-13-433
Personal Author:
Zhou, Weimin ; Wang, Wen ; Wang, Huijuan ; Lu, Roujian ; Tan, Wenjie
Zhou, Weimin ; Wang, Wen ; Wang, Huijuan ; Lu, Roujian ; Tan, Wenjie Less -
Description:
Background

Non-severe acute respiratory syndrome (non-SARS)-related human coronaviruses (HCoVs), including HCoV-229E, -HKU1, -NL63, and -OC43, have been detected in respiratory tract samples from children and adults. However, the natural prevalence of antibodies against these viruses in serum among population is unknown.

Methods

To measure antibodies to the spike (S) protein of the four common non-SARS HCoVs, recombinant S proteins of the four HCoVs were expressed and characterised in 293 T cell. An S-protein-based indirect immunofluorescence assay (IFA) was then developed to detect anti-S IgG and IgM for the four individual HCoVs and applied to serum samples from a general asymptomatic population (218 children and 576 adults) in Beijing.

Results

Of 794 blood samples tested, only 29 (3.65%) were negative for anti-S IgG. The seropositivity of the four anti-S IgG antibodies was >70% within the general population. The majority of seroconversions to four-HCoV positivity first occurred in children. Both S-IgG and S-IgM antibodies were detectable among children and increased with age, reaching a plateau at 6 years of age. However, no anti-S IgM was detected in healthy adults.

Conclusion

Large proportions of children and adults in Beijing have evidence of anti-S IgG against four the HCoVs, and first infections by all four non-SARS HCoVs takes place during childhood.


Subject:
[+]
Adolescent Adult Aged Antibodies, Viral Antibody Child Child, Preschool Coronavirus Coronavirus Infections Female Humans Infant Male Middle Aged Young Adult
Source:
BMC Infect Dis. 2013; 13:433.
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:1803a485de31a045c0cd087b3cbe56cf8845a5da8676b860f95bd9d43316d7d2
Download URL:
https://stacks.cdc.gov/view/cdc/29291/cdc_29291_DS1.pdf
File Type:

Supporting Files

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