Details:

Alternative Title:
PLoS Negl Trop Dis
Publisher's site:
http://dx.doi.org/10.1371/journal.pntd.0003580
Personal Author:
Cleton, Natalie B. ; Godeke, Gert-Jan ; Reimerink, Johan ; Beersma, Mathias F. ; van Doorn, H. Rogier ; ... More +
Cleton, Natalie B. ; Godeke, Gert-Jan ; Reimerink, Johan ; Beersma, Mathias F. ; van Doorn, H. Rogier ; Franco, Leticia ; Goeijenbier, Marco ; Jimenez-Clavero, Miguel A. ; Johnson, Barbara W. ; Niedrig, Matthias ; Papa, Anna ; Sambri, Vittorio ; Tami, Adriana ; Velasco-Salas, Zoraida I. ; Koopmans, Marion P. G. ; Reusken, Chantal B. E. M. Less -
Description:
Background

The family Flaviviridae, genus Flavivirus, holds many of the world’s most prevalent arboviral diseases that are also considered the most important travel related arboviral infections. In most cases, flavivirus diagnosis in travelers is primarily based on serology as viremia is often low and typically has already been reduced to undetectable levels when symptoms set in and patients seek medical attention. Serological differentiation between flaviviruses and the false-positive results caused by vaccination and cross-reactivity among the different species, are problematic for surveillance and diagnostics of flaviviruses. Their partially overlapping geographic distribution and symptoms, combined with increase in travel, and preexisting antibodies due to flavivirus vaccinations, expand the need for rapid and reliable multiplex diagnostic tests to supplement currently used methods.

Goal

We describe the development of a multiplex serological protein microarray using recombinant NS1 proteins for detection of medically important viruses within the genus Flavivirus. Sera from clinical flavivirus patients were used for primary development of the protein microarray.

Results

Results show a high IgG and IgM sensitivity and specificity for individual NS1 antigens, and limited cross reactivity, even within serocomplexes. In addition, the serology based on this array allows for discrimination between infection and vaccination response for JEV vaccine, and no cross-reactivity with TBEV and YFV vaccine induced antibodies when testing for antibodies to other flaviviruses.

Conclusion

Based on these data, multiplex NS1-based protein microarray is a promising tool for surveillance and diagnosis of flaviviruses.


Subject:
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Research Article

Source:
PLoS Negl Trop Dis. 9(3).
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:a7e5bc3eec55db297bdbdb78e26ff7ff4752c5f7e31d48103b39418b3500150a
File Type:

Supporting Files

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