Singlet oxygen Triplet Energy Transfer based imaging technology for mapping protein-protein proximity in intact cells

Details

• Alternative Title:
  Nat Commun
• Personal Author:
  To, Tsz-Leung ; Fadul, Michael J. ; Shu, Xiaokun
• Description:
  Many cellular processes are carried out by large protein complexes that can span several tens of nanometres. Whereas forster resonance energy transfer has a detection range of <10 nm, here we report the theoretical development and experimental demonstration of a new fluorescence-imaging technology with a detection range of up to several tens of nanometres: singlet oxygen triplet energy transfer. We demonstrate that our method confirms the topology of a large protein complex in intact cells, which spans from the endoplasmic reticulum to the outer mitochondrial membrane and the matrix. This new method is thus suited for mapping protein proximity in large protein complexes.
• Subjects:
  Article Energy Transfer Protein Binding Proteins Reactive Oxygen Species Saccharomyces Cerevisiae Singlet Oxygen
• Source:
  Nat Commun. 5:4072
• Pubmed ID:
  24905026
• Pubmed Central ID:
  PMC4091638
• Document Type:
  Journal Article
• Funding:
  DP2 GM105446/GM/NIGMS NIH HHS/United States ; T32 HL007731/HL/NHLBI NIH HHS/United States ; 1DP2GM105446/DP/NCCDPHP CDC HHS/United States
• Volume:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:9f7f399387ed069079078d5994ac29c62b2e3889741a0bb6bcc2607e7e29d644
• Download URL:
  https://stacks.cdc.gov/view/cdc/27890/cdc_27890_DS1.pdf
• File Type:
  [PDF - 2.09 MB ]