Details:

Alternative Title:
Nat Commun
Personal Author:
To, Tsz-Leung ; Fadul, Michael J. ; Shu, Xiaokun
To, Tsz-Leung ; Fadul, Michael J. ; Shu, Xiaokun Less -
Description:
Many cellular processes are carried out by large protein complexes that can span several tens of nanometres. Whereas forster resonance energy transfer has a detection range of <10 nm, here we report the theoretical development and experimental demonstration of a new fluorescence-imaging technology with a detection range of up to several tens of nanometres: singlet oxygen triplet energy transfer. We demonstrate that our method confirms the topology of a large protein complex in intact cells, which spans from the endoplasmic reticulum to the outer mitochondrial membrane and the matrix. This new method is thus suited for mapping protein proximity in large protein complexes.
Subject:
[+]
Article Energy Transfer Protein Binding Proteins Reactive Oxygen Species Saccharomyces Cerevisiae Singlet Oxygen
Source:
Nat Commun. 5:4072
Pubmed ID:
24905026
Pubmed Central ID:
PMC4091638
Document Type:
Journal Article
Funding:
DP2 GM105446/GM/NIGMS NIH HHS/United States ; T32 HL007731/HL/NHLBI NIH HHS/United States ; 1DP2GM105446/DP/NCCDPHP CDC HHS/United States
DP2 GM105446/GM/NIGMS NIH HHS/United States ; T32 HL007731/HL/NHLBI NIH HHS/United States ; 1DP2GM105446/DP/NCCDPHP CDC HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:9f7f399387ed069079078d5994ac29c62b2e3889741a0bb6bcc2607e7e29d644
Download URL:
https://stacks.cdc.gov/view/cdc/27890/cdc_27890_DS1.pdf
File Type:

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