Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy

Prevedel, Robert; Yoon, Young-Gyu; Hoffmann, Maximilian; Pak, Nikita; Wetzstein, Gordon; Kato, Saul; Schrödel, Tina; Raskar, Ramesh; Zimmer, Manuel; Boyden, Edward S.; Vaziri, Alipasha

doi:10.1038/nmeth.2964

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Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy

7 2014
By Prevedel, Robert ; Yoon, Young-Gyu ; Hoffmann, Maximilian ; ...
http://dx.plos.org/10.1038/nmeth.2964
Source: Nat Methods. 11(7):727-730

[PDF-1.34 MB]

English

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Alternative Title:

Nat Methods
Personal Author:

Prevedel, Robert ; Yoon, Young-Gyu ; Hoffmann, Maximilian ; Pak, Nikita ; Wetzstein, Gordon ; ... More +

Prevedel, Robert ; Yoon, Young-Gyu ; Hoffmann, Maximilian ; Pak, Nikita ; Wetzstein, Gordon ; Kato, Saul ; Schrödel, Tina ; Raskar, Ramesh ; Zimmer, Manuel ; Boyden, Edward S. ; Vaziri, Alipasha Less -
Description:

High-speed large-scale 3D imaging of neuronal activity poses a major challenge in neuroscience. Here, we demonstrate intrinsically simultaneous functional imaging of neuronal activity at single neuron resolution for an entire | as well as for the whole-brain of larval zebrafish. Our technique captures dynamics of spiking neurons in volumes of ~700 |m x 700 |m x 200 |m at 20 Hz and its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
Source:

Nat Methods. 11(7):727-730
Pubmed Central ID:

PMC4100252
Document Type:

Journal Article
Funding:

KL2 RR025015/RR/NCRR NIH HHSUnited States/ ; T42OH008433/ACL HHSUnited States/ ; 2T42OH008433-06/OH/NIOSH CDC HHSUnited States/ ; 5KL2RR025015/RR/NCRR NIH HHSUnited States/

KL2 RR025015/RR/NCRR NIH HHSUnited States/ ; T42OH008433/ACL HHSUnited States/ ; 2T42OH008433-06/OH/NIOSH CDC HHSUnited States/ ; 5KL2RR025015/RR/NCRR NIH HHSUnited States/ Less -
Volume:

11
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7
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CDC Public Access
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urn:sha-512:0a3a804e479d49b0b26e87c3cdb914022de44ad1802a22301d4073f4927d2cd791e7fea5262ea6df3cab40c53340ecffa569dd9b55c3f638227e3c373c58be0e
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https://stacks.cdc.gov/view/cdc/27880/cdc_27880_DS1.pdf
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