Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy

Details

• Alternative Title:
  Nat Methods
• Personal Author:
  Prevedel, Robert ; Yoon, Young-Gyu ; Hoffmann, Maximilian ; Pak, Nikita ; Wetzstein, Gordon ; Kato, Saul ; Schrödel, Tina ; Raskar, Ramesh ; Zimmer, Manuel ; Boyden, Edward S. ; Vaziri, Alipasha
• Description:
  High-speed large-scale 3D imaging of neuronal activity poses a major challenge in neuroscience. Here, we demonstrate intrinsically simultaneous functional imaging of neuronal activity at single neuron resolution for an entire | as well as for the whole-brain of larval zebrafish. Our technique captures dynamics of spiking neurons in volumes of ~700 |m x 700 |m x 200 |m at 20 Hz and its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
• Source:
  Nat Methods. 11(7):727-730
• Pubmed Central ID:
  PMC4100252
• Document Type:
  Journal Article
• Funding:
  KL2 RR025015/RR/NCRR NIH HHSUnited States/ ; T42OH008433/ACL HHSUnited States/ ; 2T42OH008433-06/OH/NIOSH CDC HHSUnited States/ ; 5KL2RR025015/RR/NCRR NIH HHSUnited States/
• Volume:
  11
• Issue:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:0a3a804e479d49b0b26e87c3cdb914022de44ad1802a22301d4073f4927d2cd791e7fea5262ea6df3cab40c53340ecffa569dd9b55c3f638227e3c373c58be0e
• Download URL:
  https://stacks.cdc.gov/view/cdc/27880/cdc_27880_DS1.pdf
• File Type:
  [PDF - 1.34 MB ]