Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia pseudomallei and Burkholderia mallei

Details

• Alternative Title:
  Am J Trop Med Hyg
• Personal Author:
  Duval, Brea D. ; Elrod, Mindy G. ; Gee, Jay E. ; Chantratita, Narisara ; Tandhavanant, Sarunporn ; Limmathurotsakul, Direk ; Hoffmaster, Alex R.
• Description:
  Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.
• Subjects:
  Antibodies, Monoclonal Burkholderia Mallei Burkholderia Pseudomallei Glanders Latex Fixation Tests Melioidosis Polysaccharides, Bacterial Species Specificity Time Factors
• Source:
  Am J Trop Med Hyg. 2014; 90(6):1043-1046.
• Document Type:
  Journal Article
• Volume:
  90
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:2dd53f10c23114dc0dda1f60321823ec888f7b2559525ad0095ec01084d79427
• Download URL:
  https://stacks.cdc.gov/view/cdc/27280/cdc_27280_DS1.pdf
• File Type:
  [PDF - 456.32 KB ]