Details:

Alternative Title:
Influenza Other Respir Viruses
Publisher's site:
http://dx.doi.org/10.1111/irv.12247
Personal Author:
Zimmerman, Richard K. ; Rinaldo, Charles R. ; Nowalk, Mary Patricia ; GK, Balasubramani ; Thompson, Mark G. ; ... More +
Zimmerman, Richard K. ; Rinaldo, Charles R. ; Nowalk, Mary Patricia ; GK, Balasubramani ; Thompson, Mark G. ; Moehling, Krissy K. ; Bullotta, Arlene ; Wisniewski, Stephen Less -
Description:
Background

Respiratory tract infections are a major cause of outpatient visits, yet only a portion is tested to determine the etiologic organism. Multiplex reverse transcriptase polymerase chain reaction (MRT-PCR) assays for detection of multiple viruses are being used increasingly in clinical settings.

Methods

During January–April 2012, outpatients with acute respiratory illness (≤7 days) were tested for influenza using singleplex RT-PCR (SRT-PCR). A subset was assayed for 18 viruses using MRT-PCR to compare detection of influenza and examine the distribution of viruses and characteristics of patients using multinomial logistic regression.

Results

Among 662 participants (6 months–82 years), detection of influenza was similar between the MRT-PCR and SRT-PCR (κ = 0·83). No virus was identified in 267 (40.3%) samples. Commonly detected viruses were human rhinovirus (HRV, 15·4%), coronavirus (CoV, 10·4%), respiratory syncytial virus (RSV, 8·4%), human metapneumovirus (hMPV, 8·3%), and influenza (6%). Co-detections were infrequent (6·9%) and most commonly occurred among those <18 years old. In regression analyses, compared with non-viral illnesses, RSV and hMPV were significantly more frequent in children and less frequent in 18- to 49-year-olds than in those ≥50 years (P = 0·01), fever was more common in hMPV and influenza infections (P = 0·008), nasal congestion was more frequent in CoV, HRV, hMPV, influenza and RSV infections (P = 0·001), and body mass index was higher among those with influenza (P = 0·036).

Conclusions

Using MRT-PCR, a viral etiology was found in three-fifths of patients with medically attended outpatient visits for acute respiratory illness during the influenza season; co-detected viruses were infrequent. Symptoms varied by viral etiology.


Subject:
[+]
Adolescent
Adult
Aged
Aged, 80 And Over
Case-Control Studies
Child
Child, Preschool
Co-detection
Female
Humans
Infant
Influenza
Male
Middle Aged
Multiplex Polymerase Chain Reaction
Original Articles
Outpatients
Prevalence
Respiratory Tract Infections
Reverse Transcriptase Polymerase Chain Reaction
RT-PCR
Viral Infections
Virus Diseases
Viruses
Young Adult

Source:
Influenza Other Respir Viruses. 2014; 8(4):397-405.
Document Type:
Journal Article
Funding:
UL1 RR024153/RR/NCRR NIH HHS/United States ; UL1 RR024153/RR/NCRR NIH HHS/United States ; UL1 TR000005/TR/NCATS NIH HHS/United States ; UL1TR000005/TR/NCATS NIH HHS/United States
UL1 RR024153/RR/NCRR NIH HHS/United States ; UL1 RR024153/RR/NCRR NIH HHS/United States ; UL1 TR000005/TR/NCATS NIH HHS/United States ; UL1TR000005/TR/NCATS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:3e8edce53ef77e71b7c5d1233d45b5cc17ab83aaa4054aa8d81ced90406e4421
File Type:

Supporting Files

• 	irv0008-0397-f1.gif	gif
  	irv0008-0397-f1.jpg	jpeg
  	irv0008-0397.nxml	txt
  	license.txt	txt

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