Tungsten carbide-cobalt nanoparticles induce reactive oxygen species, AKT, ERK, AP-1, NF-kB, VEGF, and angiogenesis.

Details

• Personal Author:
  Bowman L ; Castranova, Vincent ; Ding M ; Jiang B-H ; Zhao J
• Description:
  Powder mixtures of tungsten carbide and metallic cobalt (WC-Co) are widely used in various products. Nanoparticles are engineered structures with at least one dimension of 100 nanometers or smaller. WC-Co is known to be associated with lung injury and diseases. However, the cellular effects of WC-Co remain to be elucidated. In this study, we showed that WC-Co nanoparticles at 5 ug/cm2 induced reactive oxygen species (ROS) production which activated AKT and ERK signal pathways in a lung epithelial cell line. ROS played a key role in WC-Co induced transcriptional activation of AP-1, NF-kB,VEGF, AKT and ERK1/2; while the fine size of WC-Co particles at the same concentration did not have much effect. However, at higher concentrations, fine-size WC-Co particles also induced ROS production, AKT and ERK1/2 activation. WC-Co nanoparticles also affected the cells to induce angiogenesis. These results identify multiple signaling molecules that are induced by WC-Co nanoparticle treatment, and elucidate the potential molecular mechanism of their regulation in the cells. This information may be useful for preventing potential damage from WC-Co particle exposure in the future. [Description provided by NIOSH]
• Subjects:
  Antioxidants Lung Diseases Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Tungsten-compounds; Nanotechnology; Lung-disease; Lung-disorders; Respiratory-system-disorders; Pulmonary-system-disorders; Antioxidants
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  24
• NIOSHTIC Number:
  nn:20036760
• Citation:
  FASEB J 2010 Apr; 24(Meeting Abstracts):833.7
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal. Experimental Biology 2010, April 24 - 28, 2010, Anaheim, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/248062/cdc_248062_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]